窦神经压力感受器聚类分析

目的探讨应用Spike2软件从颈动脉窦压力感受器多重纤维放电记录中提取单纤维放电信息的可能性和具体方法。方法对离体条件下同步记录的家兔颈动脉窦压力和窦神经少纤维放电数据,应用Spike2软件的聚类分析功能对所有动作电位进行分类。结果①分析过程中,设定模板参数必须紧密结合压力感受器的自身特点;②对于具有较高信噪比的、简单(含2-3个单位)或稍复杂的窦神经放电(含5个单位),应用Spike2进行分析可获得可靠的单位放电结果;③对于包含更多单位的压力感受器放电,应用Spike2分析时,对所得结果需持审慎的态度。结论Spike2软件适用于窦神经压力感受器少纤维(<5个单位)放电的聚类分析;正确分析结果取决于原始记录良好的信噪比和对模版参数有针对性的设置。

Cluster Analysis on Carotid Sinus Baroreceptor Discharge

Objective To investigate if it is possible to extract the information of single nerve fiber discharges from multiple fiber discharges of carotid sinus baroreceptors with Spike 2 software and to establish a detailed method. Methods To record the pressure in the carotid sinus and in vitro few fiber discharges of the sinus nerve simultaneously and then to sort all the action potentials by means of Spike 2 software. Results 1 It is necessary to define several template parameters according to the baroreceptors properties. 2 Reliable unit discharges could be extracted with Spike 2 program from the sinus nerve discharges with a high signal-to-noise ratio containing 2-3 units or more 5 units. 3 It should be prudent when analyzing the baroreceptor discharges containing much more units with this program. Conclusion Spike 2 software is suitable for cluster analysis on carotid sinus baroreceptor discharges containing units not more than 5. For an accurate sorting it is a prerequisite to obtain raw records with a fine signal-to-noise ratio and to set up the template parameters pertinently.