脑膜炎奈瑟菌的PCR快速诊断

目的建立特异、敏感、快速的脑膜炎奈瑟菌检测方法。方法应用PCR技术检测流行性脑脊髓膜炎(简称流脑)疑似病例脑脊液和血液标本中脑膜炎奈瑟菌种属(Neisseria meningitides,Nm)及各群的特异性DNA片段。结果 6例流脑疑似病人的脑脊液标本中检出5份Nm CRGA基因阳性,5份含有Nm C群SIAD(C)基因片段;血液标本中检出1份Nm CRGA基因阳性,1份含有Nm C群SIAD(C)基因片段。结论所建立方法可用于Nm的快速诊断。

Rapid PCR assay for diagnosis of neisseria meningitidis

Objective To establish a specific,sensitive and rapid method for detection of Neisseria meningitides.Method Use the PCR technique to detect neisseria meningitides was detected from cerebrospinal fluid of suspected epidemic cerebrospinal meningitis cases,joseph nye meningitis strains from blood samples and specific DNA fragments of each group.Results 5 gene positive Nm CRGA were detected from 6 suspected cerebrospinal fluid samples,in which 5 contain Nm C group SIAD(C) gene fragment.1 gene positive Nm CRGA was detected from blood samples and one had SAID(C) gene fragment.Conclusions The method we established can be used in rapid diagnosis of Nm.