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| 神经鞘瘤的schwannomin/merlin表达分析 | |
| 引用本文： | 罗其中,卞留贯,孙青芳,储呈春,沈建康,赵卫国,张天锡.神经鞘瘤的schwannomin/merlin表达分析[J].中华神经外科疾病研究杂志,2002,1(2):144-147. |
| 作者姓名： | 罗其中 卞留贯 孙青芳 储呈春 沈建康 赵卫国 张天锡 |
| 作者单位： | 1. 上海第二医科大学,附属仁济医院神经外科,上海,200001 2. 上海第二医科大学,附属瑞金医院神经外科,上海,200001 3. 海安市人民医院神经外科,江苏,海安,226600 |
| 基金项目： | 上海市科委科技启明星资助项目（00QB14024），上海市教委课题资助项目（2000QN86） |
| 摘 要： | 目的 分析S／M蛋白在神经鞘瘤中的表达。方法 用免疫组织化学、蛋白印迹法观察36例神经鞘瘤中S／M的表达。结果 所有神经鞘瘤无Ⅱ型多发神经纤维瘤病（NF2）（A－19）免疫活性。28例（78％）S／M表达量下降（与正常比小于70％），其中听神经瘤占81％（13／16）、非听神经瘤占75％（15／20）。13例（36％）（听神经瘤3例、非听神经瘤10例）中等度下降（与正常比下降35％－69％）；15例（42％）（听神经瘤10例、其它5例）表达重度下降（与正常比小于35％）。结论 神经鞘瘤中S／M表达下降。 |
| 关 键 词： | 神经鞘瘤 schwannomin／merlin蛋白 免疫组织化学 蛋白印迹法 基因表达 |
| 文章编号： | 1671-2897(2002)01-144-04 |
| 修稿时间： | 2001年8月13日 |
The expression of schwannomin/merlin in neurilemmomas |
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| LUO Qizhong,BIAN Liuguan,SUN Qingfang,CHU Chengchun,SHEN Jiankang,ZHAO Weiguo,ZHANG Tianxi.The expression of schwannomin/merlin in neurilemmomas[J].Chinese Journal of Neurosurgical Disease Research,2002,1(2):144-147. | |
| Authors: | LUO Qizhong BIAN Liuguan SUN Qingfang CHU Chengchun SHEN Jiankang ZHAO Weiguo ZHANG Tianxi |
| Institution: | LUO Qizhong,BIAN Liuguan,SUN Qingfang,CHU Chengchun,SHEN Jiankang,ZHAO Weiguo,ZHANG TianxiDepartment of Neurosurgery,Renji Hospital,Department of Neurosurgery,Ruijin Hospital,Shanghai Second Medical University,Shanghai 200001, Department of Neurosurgery,Haian People's Hospital,Haian 226600,China |
| Abstract: | Objective To analyze the expression of schwannomin/merlin (S/M) in neurilemmomas. Methods The S/M expression in 36 neurilemmomas were observed by immunohistochemistry and western immunoblotting using anti-NF2 (A-19) IgG. Results It was found that all neurilemmomas were not immunoreactive to anti-NF2 ( A-19) IgG in immunohistochemistry. Reduced expression of S/M in 28 neurilemmomas(78%) was found less than 70% (compared with the normal brain tissue) , of which 13 were acoustic neuromas (13/16, 81% ), and 15 were non-acoustic neuromas (15/20, 75% ). The expression levels of S/M were moderately reduced (ratio0.35-0.69) in 13 neurilemmomas (36% , 3 acoustic neuromas,10 others). The ratio of S/M intensity in tumor/normal brain was markedly reduced, less than 35% in 15 neurilemmomas (15/36, 42% , 10 acoustic neuromas, 5 others). Conclusion The expression level of S/M was significantly lowered in neurilemmomas. |
| Keywords: | Neurilemmoma Expression schwannomin/merlin |
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