EB病毒DNA载量检测在门诊诊断鼻咽癌患者中的应用价值

目的探讨EB病毒DNA（EBV—DNA）载量检测在门诊诊断鼻咽癌患者中的应用价值。方法收集61例门诊就诊的可疑鼻咽癌患者，同时进行EBV—DNA载量、EBVVCA—IgA和EBVEA—IgA检测，并与病理组织切片检查作比较。结果病理组织切片检出鼻咽癌47例；EBV—DNA载量、EBVVCA—IgA、EBVEA—IgA检测的灵敏度分别为76．6％（36／47）、55．3％（26／47）和45．8％（22／48）；特异度分别为64．3％（9／14）、71．4％（10／14）和92．3％（12／13）；正确率分别为73．8％（36＋9）／61]、59．0％（26＋10）／61]和55．7％（22＋12）／61]；三种检测方法阳性检出率与病理组织切片检查比较，除EBV—DNA载量检测差异无统计学意义（P〉0．05）外，其余两法均低于病理组织切片检查（P均〈0．01）；EBV-DNA载量、EBVVCA—IgA和EBVEA-I吕A三种方法检测在NPC组和对照组及NPC临床分期的中位滴度，除EBV-DNA载量检测差异有统计学意义（P〈0．01）外，其他两法差异均无统计学意义（P均〉0．05）。结论在这三种鼻咽癌的筛查技术中，EBV—DNA载量检测优于EBVVCA—IgA和EBVEA—IgA检测。

The value of Epstein-Barr virus DNA load detection in the outpatient with NPC

Objective To study the value of Epstein-Barr virus DNA load detection in the outpatient with nasopharyngeal carcinoma（NPC）. Methods Sixty-one outpatients with suspected NPC were detected respectively EBV-DNA load, EBV VCA-IgA and EA-IgA, and compared with histopathological diagnosis. Results Those cases were detected 47 cases of nasopharyngeal carcinoma through histopathology. The sensitivities of EBV-DNA load, VCA-IgA and EA-IgA were 76. 6% （ 36/47 ）, 55.3 % （ 26/47 ） , and 45.8 % （ 22/48 ） respectively ; the specificities were 64. 3 % （9/14 ）, 71.4% （ 10/14 ）, and 92. 3 % （ 12/13 ） respectively ; the accuracies were 73.8 % （ 36 ＋ 9 ） / 61 ] ,59.0% （26 ＋ 10）/61 ], and 55.7% （22 ＋ 12）/61 ] respectively. Compared with histopathology, the positive rate of the three detection methods except EBV-DNA load showed no significant difference（P 〉 0. 05 ）, the other weresignificant difference（P 〈0.01 ）; The methods of EBV-DNA load, VCA-IgA and EA-IgA were performed between NPC and control groups, and among NPC clinical stages, except that the detection of EBV-DNA load was significant difference （ P 〈 0. 01 ）, the other were no significant difference （ P 〉 0. 05 ）. Conclusion In three kinds of nasopha- ryngeal cancer screening technology, EBV-DNA load was better than EBV VCA-IgA and EA-IgA.