反相高效液相色谱法同时测定乌头不同部位中新乌头碱和乌头碱含量

目的建立RP-HPLC同时测定中药乌头不同部位中新乌头碱和乌头碱的含量。方法采用Kromasil C18柱（150 mm×4.6 mm,5μm）,以40 mmol乙酸铵缓冲液-乙腈溶液为流动相,梯度洗脱,流速1.0 mL.min-1,检测波长230 nm,柱温30℃。结果新乌头碱、乌头碱分别在2.61～15.66μg、0.26～1.56μg呈良好的线性关系,加样回收率分别为100.4%（RSD=1.2%）,98.9%（RSD=0.71%）。结论该方法简便、准确,分离效果好,可用于附子中酯型生物碱的定量分析。

Determination of chemical constituents in different parts of Radix Aconiti Lateralis Preparata by RP-HPLC

Objective To develop a high performance liquid chromatography（HPLC） method for the determination of 2 principal alkaloids in different parts of Radix Aconiti Lateralis Preparata.Methods The two constituents were analyzed simultaneously with a Kromasil C18column（150 mm×4.6 mm,5 μm） by gradient elution using 40 mmol ammonium acetate buffer solution-acetonitrile as the mobile phase.The flow rate was 1.0 mL.min-1.The detection wavelenght was 230 nm.The column was maintained at 30 ℃.Results The linear ranges of the 2 constituents were 2.61-15.66 μg and 0.26-1.56 μg.The extraction recoveries were 100.4%,RSD = 1.2%,and 98.9%,RSD = 0.71%.Conclusion The method is simple,fast,and effective,which can be used to control the quality of Radix Aconiti Lateralis Preparata.