白念珠菌生物被膜耐药株麦角甾醇检测的方法学研究

目的：分别采用高效液相色谱法和气相色谱质谱联用法测定白念珠菌麦角甾醇含量,并对这两种方法进行比较。方法：采用浓度梯度递增法诱导白念珠菌耐药株,采用KONT真菌显色MIC药敏系统鉴定耐药性。构建6、12、24h生物被膜,采用高效液相色谱法和气相色谱质谱联用法测定麦角甾醇含量。结果：高效液相色谱法检测出麦角甾醇含量,最低检出浓度为0.05mg/L,而气相色谱质谱联用法未检测出麦角甾醇含量。结论：高效液相色谱法能准确测定白念珠菌麦角甾醇含量。与气相色谱质谱联用法比较,高效液相色谱法简单、高效和可靠。

A methodological study on ergosterol determination from resistant strains of Candida albicans biofilm

Objective：To determine Candida albicans biofilm ergosterol with high performance liquid chromatography（ HPLC） and gas chromatography-mass spectrometry（ GC-MS） methods,and to compare the two methods.Methods：Candida albicans fluconazole-resistant strains were induced by Candida albicans SC5314 with gradually increased fluconazole concentration,and identified by the KONT MIC determination system.Biofilm was formed at 6,12,24h.The concentration of ergosterol was determined with HPLC and GC-MS methods.Results：Ergosterol concentration was detected with HPLC,and the concentration of lowest sensitivity is0.05 mg/L,but it was not detected successfully by GC-MS.Conclusions：HPLC was an ideal way to determine the Candida albicans ergosterol concentration.