| 结核分枝杆菌rps12基因的克隆及核酸序列的分析 |
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| 引用本文： | 丁淑琴,王强,王淑静,王洁,张焱.结核分枝杆菌rps12基因的克隆及核酸序列的分析[J].宁夏医科大学学报,2011(8):729-731. |
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| 作者姓名： | 丁淑琴 王强 王淑静 王洁 张焱 |
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| 作者单位： | 宁夏医科大学; |
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| 基金项目： | 宁夏医科大学校级项目(XM200825,XM201012) |
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| 摘 要： | 目的扩增结核分枝杆菌rps12基因,并将其克隆至质粒pGEMT中进行核苷酸序列特性分析。方法以结核菌标准菌株H37Rv为模板,通过PCR技术扩增出结核分枝杆菌rps12抗原基因,将其重组到pGEM-T载体后进行酶切鉴定及序列测定和生物信息学分析。结果成功扩增出结核分枝杆菌rps12抗原基因,测序表明该片段开放阅读框由375bp组成,与已发表基因核苷酸序列相比,同源性为100%,推导编码氨基酸序列同源性为99%。结论成功克隆rps12基因,经DNA测序证实,该片段阅读框完整,该基因的获得为其原核表达及相关研究奠定了基础。
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| 关 键 词： | 结核分枝杆菌 rps12基因 克隆 同源性分析 |
Cloning and Sequence Analyzing of the rps12 Gene from Mycobacterium tuberculosis |
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| DING Shu-qin,WANG Qiang,WANG Shu-jing,WANG Jie,ZHANG Yan.Cloning and Sequence Analyzing of the rps12 Gene from Mycobacterium tuberculosis[J].Journal of Ningxia Medical College,2011(8):729-731. |
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| Authors: | DING Shu-qin WANG Qiang WANG Shu-jing WANG Jie ZHANG Yan |
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| Institution: | DING Shu-qin,WANG Qiang,WANG Shu-jing,WANG Jie,ZHANG Yan(Ningxia Medical University,Yinchuan 750004) |
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| Abstract: | Objective To obtain and analyze characters and homologies of sequence rps12 gene,and to lay bases for screening candidate antigen of Mycobacterium tuberculosis.Methods The rps12 gene of Mycobacterium tuberculosis was amplified by PCR and recombined into pGEM-T vector for sequencing and analyzing.Results A DNA sequence with an open reading frame of 375 bp was amplified successfully by PCR.Compared with the DNA sequence published,the homologies were 100%,which deduced that the amino acid sequence of rps12 of ... |
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| Keywords: | Mycobacterium tuberculosis rps12 gene cloning sequencing |
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