阿托伐他汀对氧化低密度脂蛋白诱导人内皮细胞环氧化酶-2表达的影响

目的探讨阿托伐他汀对氧化低密度脂蛋白（ox-LDL）干预后人内皮细胞环氧化酶-2（COX-2）表达的影响。方法体外培养人脐静脉内皮细胞,待细胞生长到融合状态时加入ox-LDL,对照组不加任何干预,作用24 h后,分别加入不同浓度的阿托伐他汀（1、10及30μmol/L）继续培养24 h。采用逆转录聚合酶链式反应技术分别测定各组COX-2 mRNA的表达。结果Ox-LDL可诱导COX-2 mRNA的表达;不同浓度的阿托伐他汀可抑制ox-LDL诱导的COX-2 mRNA的表达,并呈浓度依赖性（P〈0.05）。结论内皮细胞源性COX-2在动脉粥样硬化斑块形成中可能起着重要的促炎作用;阿托伐他汀可抑制人脐静脉内皮细胞COX-2 mRNA的表达。

Effect of atorvastatin on cyclooxygenase-2 expression in cultured human umbilical vein endothelial cells with the ox-LDL

Objective To investigate the effects of atorvastatin on cyclooxygenase-2（COX-2） in cultured human umbilical vein endothelial cells（HUVECs） with the ox-LDL.Methods HUVECs were cultured in endothelial basal medium.Ox-LDL were added into HUVECs,when it reached the confluence state.while there was no intervention in the control group.After 24 hours,HUVECs were cocultured with the ox-LDL and atorvastatin（1 μmol/L,10 μmol/L and 30 μmol/L）for another 24 hours.Expressions of COX-2 mRNA was determinded by reverse transcription-polymeras chain reaction（ RT-PCR）. Results Expression of COX-2 mRNA could be induced by Ox-LDL. Alorvastatin of different concentrations could suppress COX-2 mRNA expression induced by ox-LDL, which had concentration-dependent character（ P 〈 0.05 ）. Conclusions Inflammatory factors COX-2 in endothelial cell are involved in the pathogenesis of atherosclerosis. Atorvastafin can decrease the expression of COX-2 mRNA in ox-LDL-induced HUVECs.