咖啡酸苯乙酯对人结肠癌PI3K/AKT信号通路的影响

目的探讨咖啡酸苯乙酯(caffeic acid phenethyl ester,CAPE)对人结肠癌Lovo细胞内PI3K/AKT信号通路的影响。方法以体外培养人结肠癌细胞Lovo为实验对象,随机分为5组:CAPE浓度分别为0、2.5、5.0、7.5、10.0μg/ml。运用MTT法检测CAPE对人结肠癌Lovo细胞的生长增殖的影响,并通过Western blotting检测PI3K/AKT信号通路相关蛋白磷酸化AKT、caspase-3和caspase-9表达的情况。计量资料采用t检验,计数资料采用χ2检验,P<0.05为差异有统计学意义。结果 CAPE以浓度和时间依赖的方式抑制人结肠癌Lovo细胞的增殖,10μg/ml的CAPE作用72 h后对Lovo细胞的抑制率达(61.28±5.15)%,明显高于2.5μg/ml作用72 h后(29.63±0.69)%的抑制率(P<0.05),也明显高于10μg/ml作用24 h后(37.84±3.05)%的抑制率(P<0.05);Western blot-ting结果显示CAPE以剂量依赖性的方式使人结肠癌Lovo细胞内磷酸化AKT的表达下降,10μg/ml组的p-AKT的蛋白相对表达量为(0.52±0.11),caspase-3、caspase-9的表达上升,10μg/ml组的caspase-3、caspase-9蛋白相对表达量分别为(1.58±0.07)、(1.23±0.12)。结论 CAPE可能通过调节PI3K/AKT信号通路中磷酸化AKT、caspase-3和caspase-9的表达以剂量依赖性的方式抑制人结肠癌Lovo细胞的增殖。

Effect of caffeic acid phenethyl ester on PI3K/AKT signal pathway in human colon cancer cells

Objective To investigate the effect of caffeic acid phenethyl ester(CAPE) on PI3K/AKT signal pathway in human colon carcinoma Lovo cells.Methods Human colorectal cancer cell line Lovo was cultured in vitro and selected as the experimental subject.It was divided into five groups,for which the concentration of CAPE was 0,2.5,5,7.5 and 10 μg/ml respectively.MTT was used to detect the effect of CAPE on the proliferation of human colon carcinoma Lovo cells and western blotting to the expression of proteins involved in PI3K/AKT signaling pathway including phosphorylated AKT,caspase-3 and caspase-9.T-test was used for measurement data,χ2test for copparison of rates,the result of P<0.05 was considered satistically significant.Results CAPE inhibited the proliferation of human colon cancer Lovo cells in a time and concentration dependent way,10 μ g/ml CAPE action 72 h after the Lovo cell inhibition rate was 61.28%,much higher than 2.5 μg/ml action 72 h after(29.63±2.69) of inhibition rate(P<0.05),and significantly higher than 10 μ g/ml role 24 h after(37.84±3.05) of inhibition rate(P<0.05);the expression of phosphorylated AKT was decreased,10 μ g/ml group of p-AKT protein relative express quantity is(0.52±0.11);while caspase-3 and caspase-9 expression increased in cells induced by CAPE in a dose-dependent manner,10 μ g/ml group of caspase-3,caspase-9 protein relative express quantity are(1.58±0.07),and(1.23±0.12).Conclusions CAPE may inhibit the proliferation of human Lovo colon carcinoma cell through regulating the expression of phosphorylated AKT,caspase-3 and caspase-9 in a dose-dependent manner.