置环出血患者子宫内膜局部MMP-9、TIMP-1的表达

目的:研究置宫内节育器(IUD)出血患者子宫内膜局部基质金属蛋白酶-9(MMP-9)和基质金属蛋白酶抑制物-1(TIMP-1)的表达变化,并分析MMP-9与雌激素受体(ER)及孕激素受体(PR)的相关性。方法:采用免疫组化SP法检测10例正常未上环妇女,24例上环正常患者,25例上环出血患者子宫内膜(正常部位、出血部位)MMP-9、TIMP-1、ER、PR的表达情况。结果:MMP-9在出血部位的表达显著高于正常部位及其他两组,TIMP-1在出血部位的表达显著低于正常部位及其他两组,ER、PR在出血局部间质的表达降低,与其他组比较有显著性,局部MMP-9的表达与ER、PR无显著相关性。结论:局部MMP-9的表达升高、TIMP-1表达下降可能是导致IUD异常出血的直接原因,ER与PR在出血局部间质的表达下降可能与IUD出血有关,MMP-9的表达变化不是由ER或PR的变化引起的。

The expression of MMP -9 and TIMP - 1 in local endometrium of woman using IUD with abnormal uterine bleeding

Objective:To investigate the focal expression of matrix metalloproteinase-9(MMP-9) and tissue inhibitor of metailoproteinase-l(TIMP-I) in endometrium of woman using IUD with abnormal uterine bleeding and the correlation between MMP-9 and estrogen receptor(ER),proaestogen receptor(PR).Methods:The expression of MMP-9,TIMP-1,ER and PR in endometrium was detected by immunohistochemistry in 10 cases of normal women without using IUD and 24 cases of normal women using IUD and 25 cases of women using IUD with abnormal uterine bleeding.Results:The expression of MMP-9 in the focal bleeding position was significantly higher than that in normal position of endometrium and the other two groups.The expression of TIMP-1 in the focal bleeding position was significantly lower than that in normal position of endometrium and the other two groups.The expression of ER and PR in stroma of focal bleeding position was degraded.Comparing with the other groups the difference was significant.The focal expression of MMP-9 was not significantly correlated with the expression of ER and PR.Conclusion:The high expression of MMP-9 and the low expression of TIMP-1 in the focal bleeding position is possibly the direct reason leading to abnormal bleeding in IUD users.The lower expression of ER and PR in the focal bleeding stroma is probably relation to abnormal bleeding in IUD users.The changed expression of MMP-9 is not caused by the changes of ER or PR.