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| 人参药材HPLC-ELSD指纹图谱研究 | |
| 引用本文： | 蔡艳,贾继明,刘兴国,郑亚杰,王贵金,郭文胜,宋剑. 人参药材HPLC-ELSD指纹图谱研究[J]. 时珍国医国药, 2011, 22(3): 742-744. DOI: 10.3969/j.issn.1008-0805.2011.03.103 |
| 作者姓名： | 蔡艳 贾继明 刘兴国 郑亚杰 王贵金 郭文胜 宋剑 |
| 作者单位： | 河北以岭医药集团研究院,河北,石家庄,050035 |
| 基金项目： | 国际科技合作计划项目，国家科技重大专项课题 |
| 摘 要： | 目的 HPLC-ELSD法建立人参药材指纹图谱。方法采用Waters Symmetry C18(4.6 mm×250 mm,5μm)色谱柱,以乙腈-水梯度洗脱,流速为1.0 ml/min,柱温25℃,气体压力25psi、增益500、加热器级别66%、漂移管温度75℃。结果以人参皂苷Rb1为参照物,通过对10批吉林通化产的不同批次的人参药材进行指纹图谱研究,标定了9个共有峰,并利用《中国药典》中药色谱指纹图谱相似度评价系统A版,计算出其相似度均在0.99以上。结论该方法可为更好地控制人参药材的内在质量提供科学依据。 |
| 关 键 词： | 人参 指纹图谱 高效液相色谱法 |
Separation and Purification of Epimedium Flavonoids in Epimedium Brevicornum Maxim With Macroporous Absorption Resin |
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| CAI Yan,JIA Ji-ming,LIU Xing-guo,ZHENG Ya-jie,WANG Gui-jin,GUO Wen-sheng,SONG Jian. Separation and Purification of Epimedium Flavonoids in Epimedium Brevicornum Maxim With Macroporous Absorption Resin[J]. Lishizhen Medicine and Materia Medica Research, 2011, 22(3): 742-744. DOI: 10.3969/j.issn.1008-0805.2011.03.103 | |
| Authors: | CAI Yan JIA Ji-ming LIU Xing-guo ZHENG Ya-jie WANG Gui-jin GUO Wen-sheng SONG Jian |
| Affiliation: | (Hebei Yiling Pharmaceutical Institute,Hebei Shijiazhuang 050035,China) |
| Abstract: | Objective To set up the crude drug fingerprint of Ginseng Radix by HPLC-ELSD. Methods Waters Symmetry C18(4.6 mm×250 mm,5 μm) Chromatogram column was used and eluted with acetonitrile-water gradient,the rate flow was 1.0 ml/min,the gas pressure was 25psi,gaining was 500,heater rank was 66%,and drifting cube temperature was 75℃. Results Using Rb1 as the object of reference,the 10 batches of the crude drugs of Tonghua in Jilin showed nine peaks in common,and the RSD of the 10 batches of the crude drugs was above 0.99. Conclusion The method offered better scientific basis for controlling the inherent quality of Ginseng Radix. |
| Keywords: | Ginseng Radix Fingerprint HPLC |
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