黄芪抗胃溃疡作用的谱效关系研究

目的 研究黄芪95%乙醇提取物的高效液相色谱（HPLC）指纹图谱与抗胃溃疡药效的相关性，初步揭示黄芪药效物质基础。方法 ig给予胃溃疡模型小鼠黄芪乙醇提取物，用灰色关联法和偏最小二乘法将HPLC指纹图谱各共有峰的峰面积与抗胃溃疡药效数据相关联，研究谱效相关性，确定黄芪抗胃溃疡作用的物质基础。结果 黄芪95%乙醇回流提取物（总提取物）对无水乙醇所致小鼠胃溃疡有一定的抑制作用，这与黄芪的敛疮生肌之功效相一致。用灰色关联的分析方法，根据关联度大小确定黄芪乙醇提取物中各成分对抗胃溃疡作用的贡献度，并应用偏最小二乘法拟合了药效和化合物峰面积的数理方程，筛选出贡献值较大的化合物。两种统计学方法综合分析表明，在HPLC-DAD指纹图谱中，成分P9和P12（毛蕊异黄酮）贡献较大；在HPLC-ELSD指纹图谱中，成分P8和P9作用较强。结论 黄芪95%乙醇提取物较其不同极性提取部位具有较强的抗胃溃疡作用，通过HPLC指纹图谱与药效相关性分析可知黄芪抗胃溃疡作用与其所含的多种成分有关。

Spectrum-effect relationship on anti-gastric ulcer effect of Astragali Radix

Objective To investigate the relationship between HPLC fingerprint of the 95% ethanol extract from Astragali Radix (EAR) and the anti-gastric ulcer efficacy and to reveal the material basis of Astragali Radix. Methods The gastric ulcer models of mice were established, and the mice were ig administered with the ethanol EAR. The peak areas of each common peak from HPLC fingerprint were correlated with the data of anti-gastric ulcer efficacy. The grey relation statistic and partial least squares (PLS) methods were used to study the spectrum-effect relationship and to find out the material basis of anti-gastric ulcer efficacy. Results The 95% ethanol EAR (total extract) had the inhibition on anhydrous ethanol-induced gastric ulcer in mice, which is consistent with the effect of myogenic sores of Astragali Radix. The contribution of various components in ethanol EAR with anti-gastric ulcer effect was dertermined according to the spectrum-effect relationship using grey relation analysis. The equations between the efficacy and common peak areas were fit by PLS method, and the compounds with greater contribution were screened. The two methods illustrated that, in HPLC-DAD fingerprint, components P9 and P12 (calycosin) had greater contribution, and in HPLC-ELSD fingerprint, P8 and P9 were the components with greater contribution. Conclusion The 95% ethanol EAR has better anti-gastric ulcer effect compared with other extracts, and the analysis on the spectrum-effect relationship indicates that the anti-gastric ulcer effect of Astragali Radix is related to the various componets in Astragali Radix.