长鞭红景天细胞悬浮培养体系优化研究

目的 优化长鞭红景天Rhodiola fastigiata细胞悬浮培养体系及生长条件。方法 研究基本培养基、激素、碳源、氮源等因素对长鞭红景天细胞生长和红景天苷产量的影响，建立高产红景天苷的细胞悬浮培养体系。结果 MS培养基＋BA 5.0 mg/L＋2, 4-D 0.1 mg/L＋蔗糖30 g/L为长鞭红景天细胞悬浮培养的最适培养条件。结论 对培养条件进行优化，为利用长鞭红景天细胞大规模生产天然活性成分奠定基础，为缓解野生长鞭红景天资源的匮乏提供可行途径。

Optimization of cell suspension culture system of Rhodiola fastigiata

Objective To optimize the cell suspension culture system and growth conditions of Rhodiola fastigiata. Methods The effects of basic medium and hormone as well as its concentration, carbon, and nitrogen sources on the growth of R. fastigiata and the yield of salidroside were investigated and the cell suspension culture system of R. fastigiata with high yield was established. Results MS + BA 5.0 mg/L + 2, 4-D 0.1 mg/L + sucrose 30 g/L was the optimal medium for obtaining cell suspension culture of R. fastigiata with high yield. Conclusion Optimization of the culture conditions could lay a good foundation for producing natural active components through a large-scale production of R. fastigiata cells and provide a feasible way for alleviating the shortage of its natural resources.