酶解辅助提取广藿香挥发油成分分析与抗肿瘤活性初探

目的:优化广藿香挥发油的提取方法并考察其抗肿瘤活性。方法:在蒸馏法提取广藿香油之前进行酶解处理,通过正交试验研究酶的用量、酶解时间、温度以及pH对广藿香油提取率的影响,优选最佳工艺。采用气-质联用(GC-MS)测定广藿香挥发油的组成以及各成分的相对百分含量。通过MTT法测定广藿香油对Hela细胞增殖活性的抑制作用。结果:酶辅助提取广藿香油的最佳条件是:酶用量为生药重量的1%,在pH 4.5,45℃条件下酶解1 h;药典法、酶解法提取的总挥发油的提取率分别为2.2220、3.1360 mg/g;两种方法提取的广藿香油抑制He-la增殖细胞活性的IC50分别为:12.2±0.46、0.36±0.03μg/mL。结论:酶解法可提高广藿香挥发油的提取率,该法提取的广藿香油抑制Hela细胞增殖的活性强于药典法提取的广藿香油。

Extraction and Analysis of the Essential Oil in Pogostemon cablin by Enzymatic Hydrolysis and Inhibitory Activity Against Hela Cell Proliferation

Objective:To optimize the extraction method of essential oil in Pogostemon cablin and analyze its inhibitory activity against Hela cell proliferation.Methods:The Pogostemon cablin was treated by hemicellulase before steam distillation.The enzyme dosage,treatment time,treatment temperature,pH were optimized through orthogonal experimental design.The components of essential oil were identified by gas chromatography-mass spectrometry(GC-MS).Inhibitory activity of patchouli oil against Hela cell proliferation was determined by MTT method.Results:The optimum extraction process was as follows: pH 4.5,temperature 45 ℃,the ratio of hemicellulase to Pogostemon cablin was 1% and enzymatic hydrolysis for 1.0 hour.Extraction ratio of the patchouli oil in steam distillation and hemicellulase extraction method was 2.222 0 mg/g,3.136 0 mg/g respectively.Patchouli oil could inhibit Hela cell proliferation.IC50 of the patchouli oil in steam distillation and hemicellulase extraction method was 12.2±0.46 μg/mL and 0.36±0.03 μg/mL respectively.Conclusion:In comparison with steam distillation method,extraction ratios of essential oil and the inhibitory activity against Hela cell proliferation can be increased by the hemicellulase extraction method.