p57kip2和cyclin E在人宫颈癌中的表达及其与临床病理及预后的关系

目的:探讨抑癌基因p57kip2和细胞周期素E(cyclin E)在宫颈癌发生发展中的作用及其与临床病理及预后的意义. 方法: 采用免疫组化二步法检测p57kip2和cyclin E在48例宫颈癌、13例宫颈上皮内瘤变(CIN)和15例正常宫颈组织中的表达. 结果: p57kip2在宫颈癌中的阳性表达率为21%,低于正常组(93%)和CIN组(77%),差异有显著性意义(P＜0.01). cyclin E在宫颈癌中的阳性表达率为77%,明显高于正常组(13%),差异有显著性意义(P＜0.01). 在宫颈癌组织中p57kip2和cyclin E的表达呈负相关(r=-0.597, P＜0.01). p57kip2的表达与病理分化程度及肿瘤直径有关(P均＜0.05);cyclin E的表达与病理分化程度及淋巴结转移有关(P＜0.01,P＜0.05). Kaplan-Meier单因素分析宫颈癌患者生存时间与p57kip2,cyclin E阳性表达有关(P＜0.01,P＜0.01). 结论: p57kip2的低表达或缺失和/或cyclin E的高表达可能在宫颈癌发生发展中起重要作用,与不良预后有关,两者在宫颈癌细胞周期调控中可能存在着负反馈调节机制.

Expressions of p57kip2 and cyclin E in human cervix carcinoma and their correlations with clinicopathology and prognosis

AIM: To investigate the effects of tumor suppressor gene p57~(kip2) and cyclin E in the genesis and progression of human cervix carcinoma and their clinicopathological and prognosticsignificances. METHODS: The expressions of p57~(kip2) and cyclin E in tumor tissues of 48 patients with cervix carcinoma and 13 cases with cervical intraepithelial neoplasia(CIN) and 15 cases with normal cervical epithelium were detected by Poly-HRP immunohistochemical technique.RESULTS: The positive expression rate of p57~(kip2) in tumor tissues of cervix carcinoma was 21%,which was lower than those in normal cervical epithelial(93%) and CIN tissues(77%),and there were significant differences(P<0.01).The positive expression rate of cyclin E in tumor(tissues) of cervix carcinoma was 77%,which was higher than that in normal cervical epithelial tissues(13%);the difference was also significant(P<0.01).In the tumor tissues,there was a significant negative correlation between cyclin E and p57~(kip2) positiveexpressions(r=-0.597,P<0.01).P57~(kip2) positive-(expression) was associated significantly with tumor cell differentiation and lesion size(P<0.05,P<0.05).Cyclin E positive(expression) was correlated significantly with tumor cell differentiation and lymph node metastasis(P<0.01,P<0.05).Kaplan-Meier estimation indicated that the survival might be related to p57~(kip2) and cyclin E positive expressions(P<0.01,P<0.01).CONCLUSION: The decreased expression or loss of p57~(kip2) and/or over-expression of cyclin E may play an important role in the genesis and progression of cervix carcinoma,and be associated with poor prognosis,which act as a negative feedback mechanism in cell cycle regulation of cervix carcinoma.