| 广东省东莞地区慢性乙型肝炎患者HBV基因型分析 |
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| 引用本文： | 张丽华,任瑞庆,叶锦俊,韩思源,陈婉婵.广东省东莞地区慢性乙型肝炎患者HBV基因型分析[J].实用肝脏病杂志,2009,12(4):262-264. |
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| 作者姓名： | 张丽华 任瑞庆 叶锦俊 韩思源 陈婉婵 |
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| 作者单位： | 1. 广东省东莞市石龙人民医院感染病科,523326
2. 广东省东莞市石龙人民医院检验科,523326 |
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| 摘 要： | 目的探讨东莞地区乙型肝炎患者HBV基因型的分布特点。方法采用PCR-微板核酸杂交-ELISA法检测HBVA—F基因型，采用荧光定量-聚合酶链反应法检测血清HBVDNA定量。结果在295例乙型肝炎患者中检出HBV基因型247例（83．7％），其中HBV基因C型占62．3％（154／247）、B型15．4％（38／247）、D型6．9％（17／247）、B／C混合型4．9％（12／247）、C／D混合型5．3％（13／247）、B／D混合型5．3％（13／247），48例未检出基因型；基因C型感染者之间无性别差异（x^2=0．043，P〉0．05）；各基因型患者血清HBVDNA水平无显著性相差，但与48例未检出基因型者比，后者HBVDNA水平明显降低（P〈0．01）。结论PCR-微板核酸杂交-ELISA法检测HBV基因型具有特异、敏感、简单和实用的特点，东莞地区乙型肝炎患者HBV基因型以C型为主，混合型比例相对较高，HBV复制强弱与病毒基因型无关。
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| 关 键 词： | 慢性乙型肝炎 基因型 核酸杂交 聚合酶链反应 |
Hepatitis B virus genotypes in patients with chronic hepatitis B in Dongguan district of Guangzhou |
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| ZHANG Lihua,REN Ruiqing,YE Jinjun,et al..Hepatitis B virus genotypes in patients with chronic hepatitis B in Dongguan district of Guangzhou[J].Journal of Clinical Hepatology,2009,12(4):262-264. |
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| Authors: | ZHANG Lihua REN Ruiqing YE Jinjun |
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| Institution: | ZHANG Lihua,REN Ruiqing,YE Jinjun,et al. Department of Infectious Diseases,Shilong People's Hospital,Dongguan 523321,Guangdong,China |
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| Abstract: | Objective To invastigate hepatitis B virus genotypes in patients with chronic hepatitis B. Methods HBV genotypes were detected by PCR nucleic acid hybridization-ELISA. The HBV DNA levels were detected by FQ-PCR. Results Out of 295 patients with HBV infection,247(83.7%) patients had their HBV DNA genotypes detected,and 154(62.3%) were genotype C,38(15.4%) were genotype B,17(6.9%) were genotype D,12(4.9%) were mix B/C,13(5. 3%) were mix C/D and 13 (5.3%) were mix B/D infection,while 48 patients had not the ge... |
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| Keywords: | Hepatitis B Genotype Nucleic acid hybridization Polymeraze chain reaction |
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