Avastin两种用药方法抑制大鼠角膜碱烧伤后早期新生血管增生的比较

目的:探讨avastin滴眼液和结膜下注射方法对SD大鼠角膜碱烧伤后早期新生血管增生的影响。方法:制作SD大鼠角膜碱烧伤动物模型,随机分成3组,A组(对照组)、B组(avastin滴眼液组)、C组(avastin结膜下注射组),每组20只。各组分别在烧伤后3,5,7,14d应用裂隙灯显微镜观察角膜新生血管(cornealneovascu-larization,CNV)增生情况,拍照并计算CNV面积变化。同时每组各处死大鼠5只,摘取角膜组织,采用免疫组织化学方法检测VEGFR-2的表达,免疫荧光法检测CD31表达。结果:A组在伤后各时段CNV增生面积均大于B,C组;B,C组伤后各个时段VEGFR-2及CD31的表达均显著低于A组,差异有统计学意义(P<0.01);而B,C两组之间CNV增生面积及伤后各时段VEGFR-2及CD31的表达差异无统计学意义(P>0.01)。结论:角膜碱烧伤后早期应用avastin滴眼液和结膜下注射均能有效地抑制CNV生成。

Comparison of Avastin's two different treatment methods to early proliferation of corneal neovascularization after alkali burn in rats

·AIM:To investigate avastin eyedrops and subconjunctival injection of avastin on corneal neovascularization(CNV)induced by corneal alkali burns(CAB)in SD rats.METHODS:Single eye corneal alkali burn model was established in 60 SD rats.The rats were divided into 3 groups randomly.Group A(control group),group B(avastin eyedrops group)and group C(subconjunctival injection of avastin group),20 rats in each group.The occurrence and development of CNV were observed termly by slit lamp microscope on the 3th,5th,7th,14th day after corneal alkali burn,and the area of CNV was calculated by computer image analysis.Then five rats of each group were killed on the 3th,5th,7th,14th day after corneal alkali burn,and the corneas were taken for Immunohistochemistry technique to detect VEGFR-2,and immunofluorescence examination to detect CD31.RESULTS:The CNV areas of group B,C was smaller than group A(P<0.01),however there is no significant difference between B and C in terms of CNV areas(P>0.01).The level of VEGFR-2 and CD31 expression in group B,C was lower than group A(P<0.01),however there is no significant difference between B and C(P>0.01).CONCLUSION:Avastin eyedrops and subconjunctival injection of avastin can effectively inhibit CNV after alkali burns.