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| MTHFR基因C677T多态性与原因不明复发性自然流产的相关性 | |
| 引用本文： | 王苏梅,贾颐舫,杨丹形,邱毅,于建春,吕雪梅,王克华,董云玲,刘锦云. MTHFR基因C677T多态性与原因不明复发性自然流产的相关性[J]. 中国妇幼保健, 2011, 26(9): 1385-1387 |
| 作者姓名： | 王苏梅 贾颐舫 杨丹形 邱毅 于建春 吕雪梅 王克华 董云玲 刘锦云 |
| 作者单位： | 山东省计划生育科学技术研究所,山东省优生技术重点实验室,山东,济南,250002 |
| 基金项目： | 山东省计划生育科技发展项目 |
| 摘 要： | 目的:检测MTHFR基因C677T多态性及其与原因不明复发性自然流产(Unexplained recurrent spontaneous abortion,URSA)的关系。方法:应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术对非孕期URSA妇女159例及127例正常对照妇女进行MTHFR基因C677T等位基因检测,并对部分标本进行测序验证,计算患者组与对照组的基因型频率以及该多态性与URSA的相关性。结果:患者组与对照组T/T纯合子频率分别为37.1%(59/159)和16.5%(21/127),患者组与对照组T等位基因频率分别为62.9%(200/318)和47.2%(120/254),两组分别比较,差异均有统计学意义(P<0.01),对TT基因型与URSA的相关性分析表明,TT基因型妇女发生URSA的相对风险增加(OR=2.978,95%CI 1.678～5.256)。结论:MTHFR基因C677T多态性可能是山东地区URSA发病的遗传风险因素。 |
| 关 键 词： | 原因不明复发性自然流产 亚甲基四氢叶酸还原酶基因 基因突变 PCR-RFLP |
Correlation between MTHFR gene C677T polymorphism and unexplained recurrent spontaneous abortion |
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| WANG Su-Mei,JIA Yi-Fang,YANG Dan-Tong et al.Family Planning Research Institute of Science , Technology,Key Laboratory of Eugenic Technology,Ji'nan ,Sh,ong,China. Correlation between MTHFR gene C677T polymorphism and unexplained recurrent spontaneous abortion[J]. Maternal and Child Health Care of China, 2011, 26(9): 1385-1387 | |
| Authors: | WANG Su-Mei JIA Yi-Fang YANG Dan-Tong et al.Family Planning Research Institute of Science Technology Key Laboratory of Eugenic Technology Ji'nan Sh ong China |
| Affiliation: | WANG Su-Mei,JIA Yi-Fang,YANG Dan-Tong et al.Family Planning Research Institute of Science and Technology,Key Laboratory of Eugenic Technology,Ji'nan 250002,Shandong,China |
| Abstract: | Objective:To detect the relationship between methylenetetrahydrofolate reductase(MTHFR) gene C677T polymorphism and unexplained recurrent spontaneous abortion(URSA). Methods:Polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) method was used to detect the C677T allele of MTHFR gene in 159 women with URSA during non-pregnancy and 127 normal women,partial of the samples received gene sequencing,the frequencies of genotype in case group and control group were calculated,the correlation... |
| Keywords: | Unexplained recurrent spontaneous abortion Methylenetetrahydrofolate reductase gene Gene mutation PCR-RFLP |
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