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| 获取高质量生物样品图象的电镜技术浅析 | |
| 引用本文： | 章潛才,伦璇,李济和. 获取高质量生物样品图象的电镜技术浅析[J]. 中国现代应用药学, 1988, 0(4) |
| 作者姓名： | 章潛才 伦璇 李济和 |
| 作者单位： | 华南农业大学中心实验室(章潛才，伦璇)，华南农业大学中心实验室(李济和) |
| 摘 要： | 获取高分辨率和高反差的生物样品电镜图象受许多因素的影响,它包括切片厚度的选择,电镜的校直合轴、加速电压的选择、以及聚焦方法和曝光时间等。除生物样品制备技术这一重要因素外,必须考虑到上述各因素间的相互关系及其综合选择、然后才有可能获得高质量的生物样品的超微结构图象。 |
| 关 键 词： | 分辨率 反差 校直合轴 |
FACTORS AFFECTING THE ELECTRON MICROSCOPY IMAGE QUALITY OF BIOLOGICAL SPECIMEN |
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| Zhang Qiancai Lun Xuan Li Jihe. FACTORS AFFECTING THE ELECTRON MICROSCOPY IMAGE QUALITY OF BIOLOGICAL SPECIMEN[J]. The Chinese Journal of Modern Applied Pharmacy, 1988, 0(4) | |
| Authors: | Zhang Qiancai Lun Xuan Li Jihe |
| Affiliation: | Central Laboratory |
| Abstract: | To get an electron microscopy image of biological specimen with high resolution and contrast, in addition that the specimen should be well prepared, some factors are also important, such as the thickness of section, the alignment of electron microrscope, accelerating voltage, focusing and exposure time etc.The relationship of these factors and their influence on the image quality are disccussed in this paper. |
| Keywords: | Resolution Contrast Alignment |
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