反义凝血酶受体基因序列对大鼠血管平滑肌细胞增殖的影响

为了研究凝血酶受体在血管平滑肌细胞增埴中的作用 ,探讨反义凝血酶受体基因序列对大鼠血管平滑肌细胞增殖的影响 ,通过培养SD大鼠胸主动脉血管平滑肌细胞 ,以3 H TdR掺入率作为评价反义凝血酶受体基因序列抑制血管平滑肌细胞增殖的指标 ;用反转录聚合酶链反应检测反义凝血酶受体基因序列抑制血管平滑肌细胞凝血酶受体mRNA的表达 ;用Western blot检测反义凝血酶受体基因序列抑制血管平滑肌细胞凝血酶受体蛋白质的表达 ;用3 H 肌醇掺入率检测反义凝血酶受体序列抑制血管平滑肌细胞磷酸肌醇代谢的影响。结果发现 ,反义凝血酶受体基因序列明显抑制血管平滑肌细胞的增殖 (与对照组相比 ,P <0 .0 5 ) ;明显抑制血管平滑肌细胞的凝血酶受体mRNA和蛋白的表达 ;明显抑制了血管平滑肌细胞磷酸肌醇代谢。提示反义凝血酶受体基因序列明显抑制血管平滑肌细胞的增殖 ;凝血酶受体反义序列明显抑制血管平滑肌细胞的增殖是通过抑制凝血酶受体基因的表达 (特别是通过抑制DNA、mRNA和蛋白的表达 ) ,抑制细胞内信号传递来完成

Antisense Sequences of Thrombin Receptor Arrest of rat Vascular Smooth Muscle Cell Proliferation

Aim To study the influence of thrombin receptor on the proliferation of rat vascular smooth muscle cells,probe into mechanism of antisense sequence of thrombin receptor restrained the proliferation of rat vascular smooth cells. Methods Take the incoporation of 3H-thymidine as the target to evaluate vascular smooth muscle cells proliferation; Antisense sequence of thrombin receptor restrained the expression of mRNA of thrombin receptorin vascular smooth cells was detected by RT-PCR; Antisense sequence of thrombin receptor restrained the expression of protein of thrombin receptor was detected by Western-blot; The influence of antisense sequence of thrombin receptor on phosphate inositol of vascular smooth cells was detected by incoporation of 3H-inositol. Results Antisense sequence of thrombin receptor restrained vascular smooth muscle cells proliferation marketedly (compared with control P<0.05),restrained expression of mRNA and protein of thrombin receptor marketedly;Also restrained metabolism of phosphate inositol in vascular smooth cells. Conclusion Antisense sequence of thrombin receptor restrained SD rat vascular smooth muscle cells proliferation marketedly; Antisense sequence of thrombin receptor restrained vascular smooth muscle cells proliferation which could arrested gene expression of thrombin receptor,restrained signal trasduction of vascular smooth muscle cells.