|
|||||
|
|
| 破骨细胞对不同底物的吸收活性比较 | |
| 引用本文： | 付应霄,唐宝定,陈昌杰,刘宗平. 破骨细胞对不同底物的吸收活性比较[J]. 蚌埠医学院学报, 2015, 39(7): 844-846. DOI: 10.13898/j.cnki.issn.1000-2200.2015.07.002 |
| 作者姓名： | 付应霄 唐宝定 陈昌杰 刘宗平 |
| 作者单位： | 1.1. 扬州大学 兽医学院, 江苏 扬州 225009;2.2. 蚌埠医学院 生物科学系, 安徽 蚌埠 233030 |
| 基金项目： | 国家自然科学基金项目(31172373)，安徽省自然科学基金项目(1508085QH172)，安徽省教育厅自然科学研究项目(kj2012z256) |
| 摘 要： | 目的:比较破骨细胞对牛皮质骨片及牙本质片的吸收活性。方法:采用25 ng/ml 巨噬细胞集落刺激因子+30 ng/ml核因子κB受体活化因子配体诱导 RAW264.7细胞分化获取破骨细胞,并分别与牛皮质骨片及牙本质片共培养。通过检测抗酒石酸酸性磷酸酶(TRAP)染色阳性细胞数、骨吸收陷窝面积,比较破骨细胞在不同底物上的骨吸收活性。结果:与不同底物共培养形成的TRAP阳性细胞数差异无统计学意义(P>0.05),而牛皮质骨片吸收陷窝的面积显著大于牙本质片(P结论:破骨细胞对牛皮质骨片的吸收活性强于牙本质片。 |
| 关 键 词： | 破骨细胞 吸收活性 牛皮质骨片 牙本质片 |
| 收稿时间： | 2015-01-06 |
Effect of osteoclasts on the absorption activity of different substrates |
|
| Affiliation: | 1.1. College of Veterinary Medicine, Yangzhou University, Yangzhou Jiangsu 225009;2.2. Department of Bioscience, Bengbu Medical College, Bengbu Anhui 233030, China |
| Abstract: | Objective:To compare the effects of osteoclasts on the absorption activities of the bovine cortical bone slices and dentine discs. Methods:Osteoclasts were harvested from RAW264. 7 cells induced by 25 ng/ml M-CSF combined with 30 ng/ml RANKL, which was cocultured with bovine cortical bone slices and dentine discs,respectively. The number of positive cells and area of resorption lacunae were detected by antitartaric acid phosphatase staining, and the absorption activities of different substrates were compared. Results:The difference of the TRAP positive cells in different substrates coculture was not statistically significant(P>0. 05). The area of resorption lacunae in bovine cortical bone slices was significantly more than that in dentine discs(P<0. 01). Conclusions:Compared with the dentine discs,the absorption activity of osteoclasts on bovine cortical bone slices is strong. |
| Keywords: | osteoclast bone resorption activity bovine cortical bone slice dentin slices |
| 本文献已被 万方数据 等数据库收录！ | |
| 点击此处可从《蚌埠医学院学报》浏览原始摘要信息 | |
| 点击此处可从《蚌埠医学院学报》下载全文 | |