毛细管气相色谱法测定羟乙基淀粉中2-氯乙醇的残留量

目的建立毛细管气相色谱法测定羟乙基淀粉中2-氯乙醇的残留量。方法采用毛细管气相色谱法。色谱柱为DM-WAX弹性石英毛细管柱(30 m×0.25 mm,0.25μm);载气为氮气;检测器为ECD;进样口和检测器温度均为250℃;柱温为程序升温,初始温度90℃,保持12 min,以每分钟20℃升温至120℃,保持2 min。结果2-氯乙醇质量浓度在14.30~119.2μg.L-1内线性关系良好,r=0.997 6;回收率为98.4%(n=9);检测限为9.54×10-3ng;定量限为2.86×10-2ng。结论本法灵敏度高,简单,易行,结果准确可靠,可为羟乙基淀粉的质量控制提供依据。

Determination of 2-chloroethanol residue in hydroxyethyl starch by capillary GC

Objective To establish a GC method for the determination of 2-chloroethanol residue in hydroxyethyl starch. Method A column of DM-WAX was used with ECD as detector; nitrogen was selected as carrier gas. The injector temperature and the detector temperature were 250 ℃; the programmed column temperature was set at 90 ℃ for 12 min and then to 120 ℃ at the rate of 20 ℃·min^-1 followed by holding for 2 min. Results A good linearity was obtained at the concentration of 14.30-119.2 µg·L^-1 with correlation of 0.997 6. The average recovery was 98.4% (n = 9), the limit of detection was 9.54×10^-3 ng; the limit of quantity was 2.86×10^-2 ng. Conclusion This method is simple, accurate and feasible. It is very suitable for the quality control of 2-chloroethanol in hydroxyethyl starch.