hTERT基因反义核酸对K562细胞端粒酶活性的影响

探讨人类端粒酶逆转录酶（ｈｕｍａｎ ｔｅｌｏｍｅｒａｓｅ ｒｅｖｅｒｓｅ ｔｒａｎｓｃｒｉｐｔａｓｅ,ｈＴＥＲＴ）基因的反义寡核苷酸（ａｎｔｉｓｅｎｓ ｏｌｉｇｏｄｅｏｘｙｎｕｃｌｅｏｔｉｄｅ,ＡＳＯＮＤ）对Ｋ５６２细胞端粒酶活性的影响。方法：采用多聚酶链反应－酶联免疫测定（ＰＣＲ－ＥＬＩＳＡ）方法检测人工合成的反义脱氧寡核苷酸处理Ｋ５６２细胞前后端粒酶活性的改变,以逆转录ＰＣＲ（ＲＴ－ＰＣＲ）分

Effect of hTERT antisense oligodeoxynucleotide on telomerase activity in K562 cells

Objective: To evaluate the effect of human telomerase reverse transcriptase (hTERT) gene antisense oligodeoxynucleotide (ASODN) on telomerase activity in K562 cells. Methods: Telomerase activity was determined by poymerase chain reaction enzyme-linked immunoassay (PCR-ELISA ) in K562 cells untreated or treated with ASODN, and hTERT mRNA expression was analyzed by reverse transcriptase polymerase chain reaction (RT-PCR). Results: The hTERT mRNA level was decreased, while telomerase activity was significantly inhibited when the K562 cells were treated with ASODN for 48h. Conclusion: It is suggested that hTERT ASODN might specifically inhibit telomerase activity of K562 cells at translation level, and it is further proved that hTERT gene has significant correlation with telomerase activity.