| 荧光定量RT—PCR检测mdr1在急性白血病中的表达及临床意义 |
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| 引用本文： | 王松梅,于建宪,徐克惠,邢秀华.荧光定量RT—PCR检测mdr1在急性白血病中的表达及临床意义[J].山东医药,2001,41(5):3-4. |
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| 作者姓名： | 王松梅 于建宪 徐克惠 邢秀华 |
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| 作者单位： | 岛市立医院 |
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| 摘 要： | 采用荧光定量RT-PCR法检测36例不同类型白血病患者的多药耐药基因（mdr1mRNA）表达，同时检测15例骨髓或外周血正常患者，以做对照。结果对照组均为阴性表达，初治患者组mdr1阳性基因拷贝数平均为2.7×10
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| 关 键 词： | 荧光定量RT－PCR法 急性白血病 mdr1基因 基因表达 |
| 修稿时间： | 2000年12月18 |
Expression and significace
of mdr1 in patients with acute leukemia |
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| Wang Songmei,Yu Jianxian,Xu Kehui,et al.Expression and significace
of mdr1 in patients with acute leukemia[J].Shandong Medical Journal,2001,41(5):3-4. |
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| Authors: | Wang Songmei Yu Jianxian Xu Kehui et al |
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| Abstract: | The expression of multi-drug resistance gene（mdr1 mRNA）wasmeasured in 36 patients with different type of acute leukemia and in 15 normal controls by fluorogenic probe quantitative RT-PCR（FQ-RT-PCR）.Result showed that the mdr1 mRNA was neg ative in normal controls． The average level of the mdr1 gene express in untreated group was 2.7×103 copies／μgRNA，while that of clinical resistance group was 1.0×104 copies／μgRNA（P＜0.05）.The CR rate differed significantly between mdr1 positive group（21％）and negative group（82％）（P＜0.05）.It suggests that FQ-RT-PCR is an acurate quantitative method in detecting the mdr1 gene expression，a high level of mdr1 expression correlated closely with drug resistance in clinical leukemia chemotherapy. |
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| Keywords: | Reverse transciption-polymerase chain reaction Acute leukemia mdr 1 gene expression |
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