KDR基因沉默对胃癌MGC-803细胞增殖和凋亡的影响

[目的]探讨KDR基因对胃癌MGC-803细胞增殖和凋亡的影响。[方法]设计及合成KDR的siRNA序列,LipofectamineTM 2000转染MGC-803细胞。通过RT-PCR、Western Blot检测KDR在干扰后的mRNA和蛋白的表达情况,利用流式细胞仪检测细胞周期,WST-1法检测细胞增殖活性,TUNEL法检测细胞凋亡情况。[结果]KDR mRNA和蛋白表达,观察组较对照组和空白组显著降低,差异有统计学意义(P<0.05)。观察组细胞的生长速度明显减慢,细胞周期被阻滞在G0/G1期,S期细胞数减少,差异有统计学意义(P<0.05)。转染KDRsiRNA的MGC-803细胞的增殖能力明显受到抑制(P<0.05),且明显促进了细胞的凋亡,差异有统计学意义(P<0.05)。[结论]特异性干扰KDR基因表达可抑制胃癌MGC-803细胞的增殖,并促进肿瘤细胞凋亡。KDR的siRNA序列可能成为治疗胃癌的有效靶点。

Effect of KDR Gene Silence on Proliferation and Apoptosis of Gastric Cancer Cell Line MGC-803

[Purpose] To explore the effect of KDR gene in the proliferation and apoptosis of gastric cancer cell MGC-803. [Methods] The siRNA of KDR was constructed and transfected into MGC-803 cells with LipofectamineTM 2000 . The expression of KDR mRNA and protein were detected by RT- PCR and Western Blot method. Flow cytometry was used to detect the cell cycle, the cell proliferation was assessed by WST-1 assay,and cell apoptosis was detected by TUNEL. [Results~ Compared with the control group and blank group ,the mRNA and protein level was significantly decreased in the ob- servation group when transfected with KDR siRNA. The growth slowed down and the cell cycle was arrested at Go/G1 phase and cell number in S phase was decreased in MGC-803 cell line （P〉0.0S）.Af- ter KDR siRNA transfection, the proliferation of MGC-803 cells was markedly inhibited and the apop- tosis of MGC-803 cells was increased （P〈0.05）. [Conclusionl Interference of KDR gene may suppress cell growth and promote cell apoptosis in MGC-803 cell line.Thus, KDR siRNA might be an effective target spot in the treatment for gastric cancer.