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Cecal ligation and puncture induced sepsis impairs host defense against Enterococcus faecium peritonitis |
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| Authors: | Masja Leendertse Rob J. Willems Ida A. Giebelen Sandrine Florquin Petra S. van den Pangaart Marc J. Bonten Tom van der Poll |
| Affiliation: | (1) Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center, Amsterdam, The Netherlands;(2) Center for Experimental and Molecular Medicine, Academic Medical Center, G2-130, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands;(3) Department of Pathology, Academic Medical Center, Amsterdam, The Netherlands;(4) Department of Medical Microbiology, University Medical Center Utrecht, Utrecht, The Netherlands;(5) Julius Center for Health Studies and Primary Care, University Medical Center Utrecht, Utrecht, The Netherlands |
| Abstract: | Purpose Multiresistant and vancomycin resistant Enterococcus faecium (VRE) can cause serious infections in hospitalized patients with various co-morbid diseases. We investigated the course of VRE peritonitis after cecal ligation and puncture (CLP)-induced sepsis and compared this to sham operated mice. Methods Mice were subjected to CLP or sham surgery. Forty-eight hours thereafter four groups were created by subjecting mice to peritoneal injection of either VRE or saline. Results Mice infected with VRE after CLP were severely impaired in eliminating VRE from the peritoneal cavity and distant body sites. These mice failed to mount an early inflammatory response at the primary site of VRE infection. VRE superinfection did not influence CLP-induced organ damage or polymicrobial bacterial loads. Conclusions Sublethal polymicrobial sepsis greatly facilitates infection and dissemination of VRE. VRE does not influence the course of CLP-induced sepsis. |
| Keywords: | Enterococcus faecium Cecal ligation and puncture (CLP) Innate immunity Immunoparalysis Sepsis Mouse model |
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