阳离子卟啉光动力疗法作用于人喉癌Hep-2细胞的实验研究

目的：探讨阳离子卟啉对人喉癌Hep-2细胞的光动力疗法(PDT)的作用。方法：采用4-甲酰基苯甲酸甲酯和4-吡啶甲醛经化学反应合成阳离子卟啉(5-[4-[1-(4-苯基哌嗪基)-乙酰氧羰基]苯基]-10，15，20-三(4-N-甲基吡啶盐)卟啉，三碘盐)。以倒置显微镜下观察细胞生长状态，应用四唑盐比色(MTT法)和原位末端标记技术(TUNEL)分别研究阳离子卟啉在不同浓度(0、10^-7、10^-6、10^-5mol／L)及不同时间(0、0．5、1．0、1．5、2．0h)高压汞灯照射下对人喉癌Hep-2细胞的生长抑制和诱导凋亡作用。结果：在一定浓度范围内，阳离子卟啉对Hep-2细胞生长增殖的抑制作用和诱导凋亡作用随着浓度和光照时间的增加而增强。最高浓度(10^-5mol／L)及不同光照时间下Hep-2细胞生长抑制率分别为1．18％、35．21％、58．59％、70．42％、84．34％，诱导的细胞凋亡率分别为3．30％、29．41％、32．30％、34．52％。大剂量和高能量照射可直接导致少量细胞坏死，随着光照时间的延长，未加卟啉的对照组对细胞生长亦有一定的抑制作用。结论：阳离子卟啉在高压汞灯的照射下能抑制体外培养的Hep-2细胞生长增殖并诱导部分Hep-2细胞凋亡，其治疗效果在一定浓度范围内与用药剂量和照射能量相关。

The photodynamic therapy effect of a new cationic porphyrin on human laryngeal cancer Hep-2 cell in vitro

OBJECTIVE: To investigate the photodynamic therapy (PDT) effect of a new kind of cationic porphyrin on human laryngeal cancer Hep-2 cell in vitro. METHOD: A new cationic porphyrin (5-(4-methoxycarbonylphenyl)-10, 15, 20-tris(4-N-methylpyridiniumyl)porphyrin triiodide ) was synthesized. The Hep-2 cells were treated with three different concentrations of porphyrin (10(-7) mol/L, 10(-6) mol/L, 10(-5) mol/L) followed by irradiation of different visible light (0, 0.5, 1.0, 1.5, 2.0 h). MTT and TUNEL were applied to measure the inhibitory effect of porphyrin on the Hep-2 cells growth, inhibitory rate and induced apoptosis rate. RESULT: Under definite condition, the cantionic porphyrin concentrations had a significant inhibitory effect and induced apoptosis effect. The effect depended greatly on the doses of the concentration and the time of irradiation of light. At the highest concentration of porphyrin (10(-5) mol/L), the growth inhibitory rate were 1.18%, 35.21%, 58.59%, 70.42%, 84.34% respectively and the apoptosis rate were 3.30% +/- 0.29%, 29.41% +/- 2.12%, 32.30% +/- 2.26%, 34.52% +/- 3.11% in different visible light (0.05, 1.0, 1.5, 2.0 h), respectively. Under high irradiation energy, it can causesome Hep-2 cells death directly. CONCLUSION: Porphyrin has a distinctive killing effect on Hep-2 cells and correlated with the doses and the irradiation time.