新型纳米纤维支架的细胞生物相容性◆

背景：高孔隙率聚己内酯纳米纤维支架具有适合血管平滑肌细胞黏附、增殖的多级孔径结构，具有良好的细胞生物相容性。 目的：探讨高孔隙率聚己内酯静电纺丝纳米纤维支架的细胞相容性。 方法：根据支架的制作工艺不同分为传统支架组、新型纳米纤维支架组两组，另设单纯细胞组为对照组。采用组织块贴壁法体外原代培养兔主动脉平滑肌细胞并进行传代，用3~6代细胞作为实验用种子细胞。应用WST-1法测定平滑肌细胞黏附率、增殖力，光镜及扫描电镜观察细胞形态，评估支架的细胞生物相容性。 结果与结论：高孔隙率聚己内酯纳米纤维支架对细胞形态无明显影响，新型支架上的种子细胞黏附、增殖及代谢活性情况较传统支架好。提示，高孔隙率聚己内酯静电纺丝纳米纤维支架具有较高的细胞相容性。

Cell biocompatibility of a new-type nanofiber scaffold for tissue engineering

BACKGROUND: High-porosity polycaprolactone (PCL) nanofiber scaffolds have a good multi-pore structure for the adhesion and proliferation of vascular smooth muscle cells and perfect biocompatibility. OBJECTIVE: To evaluate the cell biocompatibility of high-porosity PCL scaffold made by electrostatic spinning technology. METHODS: Electrostatic spinning technology was used to produce high-porosity PCL nanofiber stents. WST-1 method was used to evaluate the effects of scaffold on the adhesion and proliferation of smooth muscle cells cultured with different scaffolds. Light microscope and scanning electron microscope were used to observe the morphology of cells. RESULTS AND CONCLUSION: The high-porosity PCL scaffold exhibited better biocompatibility than the traditional one in cell morphology, adhesion and proliferation. It is indicated that the high-porosity PCL nanofiber stents produced by electrostatic spinning technology owns better biocompatibility.