The organization of the genes for ribosomal RNA on mitochondrial DNA of Kluyveromyces lactis |
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| Authors: | Gert S. P. Groot Nel Van Harten-Loosbroek |
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| Affiliation: | (1) Section for Molecular Biology, Laboratory of Biochemistry, University of Amsterdam, Animal Physiology Institute, Kruislaan 320, 1098 SM Amsterdam, The Netherlands;(2) Section for Medical Enzymology, Jan Swammerdam Institute, P. O. Box 60,000, 1005 GA Amsterdam, The Netherlands;(3) Present address: Laboratory of Biochemistry, Free University, de Boelelaan 1085, 1081 HV Amsterdam, The Netherlands |
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| Abstract: | Summary We have constructed a physical map of Kluyveromyces lactis mtDNA using the restriction enzymes HindII and HindIII. In contrast to Saccharomyces, the genes for the large and small ribosomal RNAs are much closer to each other, being separated by a maximal distance of 2,250 base pairs.Abbreviations bp base pair(s) - rRNA ribosomal RNA - SSC 0.15 M NaCl, 0.015 M sodium citrate (pH 7.0) |
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| Keywords: | K. lactis mtDNA Physical mapping Ribosomal RNA |
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