乳腺浸润性导管癌组织中HPV16DNA与p53蛋白表达的研究

 目的 检测人乳腺浸润性导管癌组织中HPV16DNA和p53蛋白，探讨HPV16型感染和p53蛋白表达与人乳腺癌之间的关系。方法 采用分子原位杂交和免疫组化技术检测和分析50例乳腺浸润性导管癌、30例正常乳腺组织中HPV16DNA和p53蛋白二者之间的表达关系。结果 癌组中HPV16DNA阳性和HPV16DNA阴性两组阃p53蛋白的表达均有显著性差异（P〈0．01），HPV16DNA阳性组显示与p53蛋白表达呈负相关（P〈0．02）。结论 HPV16感染后导致野生型p53的降解可能涉及HPV16感染后人乳腺上皮细胞癌变的病理发生过程。

Study on the Expression of HPV16 DNA and p53 Protein in Mammary Invasive Duct Carcinomas

Objective To investigate the expression of HPV16DNA and p53protein in breast cancer and explore its relation between expression of HPV16infection with p53and the carcinogenesis of breast cancer.Methods HPV16DNA and p53protein were detected by in situ hybridization and immunohisto- chemical staining in50cases of invasive duct carcinoma and30cases normal breast tissues .Results There was a significant difference in the expression of p53protein between HPV16DNA positive and HPV16DNA negative breast carcinomas groups (P<0.01) , with a significant less detectable p53pro- tein in the HPV16DNA positive cases .Conclusion Inactivation and degradation of wild type p53protein by HPV16infection in human mammary epithelial cells may play an important role in the pathogenesis human breast carcinomas.