Ⅱ度烧伤病人皮肤角朊细胞ICAM-1的表达

目的探讨Ⅱ度烧伤创面愈合过程中角朊细胞间粘附分子１（ＩＣＡＭ１）的表达及其意义。方法应用免疫组织化学染色技术，对烧伤后不同时间Ⅱ度烧伤皮肤组织角朊细胞ＩＣＡＭ１的表达进行动态观察。８例健康人皮肤，６例烧伤病人正常皮肤及８例Ⅱ度烧伤愈合后增生性瘢痕组织为对照组。结果健康人正常皮肤基底层细胞ＩＣＡＭ１可低表达，烧伤病人正常皮肤基底层细胞ＩＣＡＭ１可表达增强。Ⅱ度创面基底层细胞伤后１周内ＩＣＡＭ１表达轻度增高，２周内明显增强，２～４周稳定高表达，ＩＣＡＭ１呈极性分布，新生上皮多层角朊细胞ＩＣＡＭ１表达显著增强，ＩＣＡＭ１阳性细胞呈长柱状排列。结论Ⅱ度烧伤皮肤角朊细胞ＩＣＡＭ１表达增强可能与创基炎性细胞浸润相关，具有诱导角朊细胞增殖、迁移，加速上皮化的作用。

The expression of ICAM|1 on the keratinocytes in second degree human burn skin

Objective To investigate the significance of changes in intercellular adhesion molecule|1 (ICAM|1) expression on keratinocytes(KCs) during wound healing of second degree burn. Methods With immunohistochemistry technique,the ICAM|1 expression in second degree burn wounds in patients were investigated at four different postburn periods.The normal skins from 6 burn patients (BNG),the normal skins from 8 healthy volunteers (HVG) and the scar tissues (SG) from 8 patients served as controls. Results There was poor expression of ICAM|1 on basal cells in normal skins of healthy volunteers,while the expression of ICAM|1 enhanced in normal skins of burn patients.On basal cells in second degree burn wounds,the expression of ICAM|1 slightly increased in the first week postburn,obviously enhanced in the second week,and in the third or fourth week,the expression continued to keep strong.In addition,the ICAM|1 in KCs showed polar distribution,and the expression of ICAM|1 markedly enhanced on the multilayer KCs in neogenetic epithlium and the positive cells arranged columnarly. Conclusion The enhanced expression of ICAM|1 on KCs in second degree burn wounds might be related to invasion of inflammatory cells beneath wound.It might play a role in inducing proliferation and migration of KCs and accelerating epithelization.