N-甲基亚硝基脲的制备及其诱发膀胱肿瘤的作用

目的 研究Ｎ－甲基亚硝基脲（ＭＮＵ）制备方法及其诱发大白鼠膀胱肿瘤的作用。方法 由甲胺盐酸盐合成诱癌剂ＭＮＵ，进行大白鼠膀胱灌注２ｍｇ／次，每２周１次，共４次，以光镜、扫描电镜、透射电镜等为观察手段，对其诱发的膀胱肿瘤进行观察。结果 ８周诱发膀胱肿瘤率为１００％，其组织学检查及病理学特征与人膀胱肿瘤十分相似。结论 自制的ＭＮＵ诱发的大白鼠膀胱肿瘤为比较理想的动物模型。

Synthesis of N-methyl-N-nitrosourea(MNU)and development of experimental model of bladder tumors induced by MNU in rats

Objective To study preparation of N methyl N nitrosourea (MNU) and development of the experimental animal model of bladder tumors induced by MNU in rats. Methods Bladder tumors were induced in femal wistar rats by intravascular administration of the carcinogen, MNU that was synthesized with CH 3NH 2HCL. The morphological alterations in bladder epithelial cells were studied under light microscopy, scanning and transmission electron microscopy. Results The administration way of a total dose of 8 mg on 4 fraction of 2 mg at 2 weekly intrvals produced a 100 % incidence of bladder tumor after 8 weeks. Histopathological characteristics of bladder tumor induced by MNU were basically similar to those of human bladder tumor. Conclusion Intravascular administration of MNU in rats provided a useful model for experimental studies on bladder tumor.;