厦门市乙型肝炎患者病毒基因型的分析

目的:采用多对型特异性引物,通过巢式PCR法检测厦门市乙型肝炎患者血清中乙型肝炎病毒(HBV)基因型的分布情况.方法:收集250例HBV感染患者血清,提取血清中HBV DNA作为模板,设计HBV前S1基因和S基因中区域内设计出10条内外引物,并将其中8条型特异性内引物分成A,B两组,分别扩增A,B,C和D,E,F型HBV,然后将第2轮PCR产物以用30g/L琼脂糖进行电泳,根据PCR产物电泳显示的产物长度判定HBV基因型,以了解厦门HBV基因型分布情况.结果:共120例确定了HBV基因型.患者群中慢性乙型肝炎90例,占75.0%,急性乙型肝炎、肝炎肝硬化、原发性肝癌分别占5.8%(7/120)、6.7%(8/120)和12.5%(15/120).分型结果:B型58例(48.3%)、C型30例(25.0%),B/C混合型32例(26.7%).HBeAg阳性患者中B基因型占63.8%,B/C型混合感染21.9%;抗-HBe阳性患者中以B/C型混合感染68.8%,B型25.9%,HBeAg阳性组与抗-HBe组之间比较发现B型和B/C混合型之间(P<0.05).结论:厦门乙型肝炎患者HBV基因型以B型为主,B/C混合感染是一个值得重视的问题.

Preliminary study on hepatitis B virus genotypes in Xiamen city

AIM:To investigate the genotypes of hepatitis B virus(HBV)in Xiamen city by nested polymerase chain reaction(PCR)with multiplex pairs of genotype-specific primers. METHODS:A total of 250 HBV-infected patients were included in this study.The serum samples were collected and the serum HBV DNA was used as templates.Ten outer and inner prim- ers were designed on the basis of nucleotide sequences in the regions of Pre-S1 and S genes, of which 8 genotype-specific inner primers were divided into 2 groups:A and B.Genotype A, B,C or D,E,F of HBV were amplified,respec- tively.The genotypes of the second-round PCR products were identified using agarose gel elec- trophoresis(30 g/L). RESULTS:Of the 250 selected patients,120 re- ceived the above genotyping successfully.There were 90(75.0%)diagnosed with chronic hepa- titis B,7(5.8%)with acute hepatitis B,8(6.7%) with liver cirrhosis,and 15(12.5%)with hepato- cellular carcinoma.Of the 120 cases,58(48.3%) were found with genotype B,30(25.0%)with genotype C and 32(26.7%)with genotype B/C. In the patients with positive HBeAg,genotype B and B/C were confirmed in 63.8% and 21.9% of them,respectively.However,the patients with genotype B/C and B accounted for 68.8% and 25.9% of the anti-HBe-positive patients.There were significant differences between the 2 sub- groups in the genotype distribution(P<0.05). CONCLUSION:Genotype B prevails among HBV-infected patients in Xiamen city,and type B/C HBV infection a considerable problem.