EGFR抑制剂吉非替尼对食道癌细胞的抑制作用

目的　探讨EGFR抑制剂吉非替尼对人食管癌细胞Eca-109的抑制作用。方法　以不同浓度吉非替尼作用于Eca-109细胞,MTT法检测在不同作用时间( 24、48、72h)细胞生存率；流式细胞仪检测不同浓度的吉非替尼作用48h后Eca-109细胞周期的变化。结果　MTT结果显示吉非替尼不同浓度不同时间生存率差异均有显著性(均P <0.05) 。同一浓度下不同作用时间组生存率差异均有显著性(均P < 0.05) ,而24h不同浓度处理后细胞的生存率具有显著差异(均P < 0.05)，48、72h除40ug/mL与80ug/mL无显著差异（P>0.05）外,其余的浓度组间差异均有显著性(均P < 0.05) 。细胞周期结果示吉非替尼各浓度组均使细胞周期阻滞于G0/G1期，与对照组相比有显著性差异(P<0.05)；各浓度组之间比较，高浓度组较低浓度组具有显著差异性(P<0.05)。同时伴随着S,G2/M期细胞比例的下降，部分组间差异具有统计学差异(P<0.05)。结论 吉非替尼可能通过阻滞食管癌细胞周期于G0/G1期，从而抑制食管癌细胞生长。

Gefitinib, an epidermal growth factor receptor blockade agent, shows effects on the antiproliferation of esophageal cancer cells in vitro.

Objective　To investigate the effect of gefitinib, an epidermal growth factor receptor blockade agent, on the proliferation of esophageal cancer cells Eca-109 in vitro. Methods　Human Eca-109 cells were dealed with Gefitinib in different concentration, Survival rate of each concentration was measured by MTT assay after 24,48,72hours. The cell cycle was measured with flow cytometric method(FCM)．Results　The cell survival rate between different concentration and different time were all significantly different(P <0.05). The cell survival rate were all significantly different (P <0.05) under the same concentration.Afrter 24hours, The cell survival rate in different concentration was also significantly different in contrast to the contol group.The cell survival rate was also significantly different with different concentration in 48, 72hours except that the concentration was 40ug/ml and 80ug/ml ( all P < 0. 05). Gifitinib in different concentrention can induced accumulation of cells in G0/ G1 phase compared with untreated control (P<0.05)；and the distinctions in diffirent gifitinib groups are apparent( all P < 0. 05).Accordingly ,the number of cells in the S-phase and G2/M -phase are decreased. The differences in some groups are evident.Conclusion The present study suggested that gifitinib may inhibit the proliferation of human esophageal cancer cell lines in vitro efficiently. The mechanism, perhaps , may be related to an arrest effect on cell cycle in G0/G1 phase .