| 玻璃纤维诱导的转化细胞活化癌基因的研究 |
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| 引用本文： | 高宏光 王国荃. 玻璃纤维诱导的转化细胞活化癌基因的研究[J]. 毒理学杂志, 1992, 6(4): 261-263 |
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| 作者姓名： | 高宏光 王国荃 |
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| 作者单位： | 广州医学院化学致癌研究所，新疆医学院环境卫生学教研室，美国国家职业安全与卫生研究所，美国国家职业安全与卫生研究所 广州 510182，乌鲁木齐 830054，Morgantown，WV26505，Morgantown，WV26505 |
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| 摘 要： | 用玻璃纤维处理BALB/C3T3细胞,使细胞发生转化,从转化细胞中抽提DNA用以转染NIH3T3细胞,结果被转染的细胞亦发生转化。用~(32)P标记的Ha-ras癌基因作探针,通过斑点杂交,征明玻璃纤维诱发的BALB/3T3转化细胞中,Ha—ras的原癌基因被激活。
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| 关 键 词： | 玻璃纤维 转化 转染 癌基因 |
The Observation of Oncogene Activation in Transformed Cells Induced by Glass Fibers |
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| Gao Hong-guang,et al.. The Observation of Oncogene Activation in Transformed Cells Induced by Glass Fibers[J]. Journal of Toxicology, 1992, 6(4): 261-263 |
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| Authors: | Gao Hong-guang et al. |
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| Abstract: | Studies have been performed in our laboratory to determine whether three glass fibers, JM 100, owens corning AAA-10, and owens corning general building insulation fibers can induce morphological transformation in BALB/c3T3 cells. DNA from transformation cells were isolated and analyzed for oncogene activation. The results showed that reaction in vitro with activated oncogene, c-Ha-ras, when the transformed cell DNA was introduced into NIH3T3 cells, c-Ha-ras oncogene were expressed in the transformed cells and formed the morphological tra-nsformation of NIH3T3 cells. These characterization for the transformation indicated that c-Ha-ras oncogene were activated in the NIH3T3 cells and performed malignant phenotype. |
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| Keywords: | Glass fibers Transformation Transfection Oncogene. |
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