藏边大黄的化学成分研究

目的研究藏边大黄的化学成分。方法用硅胶、聚酰胺柱色谱分离结合重结晶方法进行分离纯化。用MS、1H-NMR、13C-NMR谱学方法结合化学方法确定化合物的结构。结果从藏边大黄干燥根与根茎的乙醇提取物中分得18个化合物,鉴定了其中16个化合物的结构,分别为大黄酚(1)、大黄素甲醚(2)、β-谷甾醇(3)、大黄素(4)、芦荟大黄素(5)、波叶素(6)、胡萝卜苷(7)、d-儿茶素(8)、反式-3,5,3′,4′-四羟基茋(9)、反式-3,5,3′,4′-四羟基茋-4′-O-β-D-吡喃葡萄糖苷(10)、反式-3,5,3′,4′-四羟基茋-4′-O-β-D-(6″-O-p-香豆酰)-吡喃葡萄糖苷(11)、大黄酚-8-O-β-D-吡喃葡萄糖苷(12)、大黄素甲醚-1和8-O-β-D-吡喃葡萄糖苷(13a和13b)、大黄素-8-O-β-D-吡喃葡萄糖苷(14)、蔗糖(15)。同时得到一种大黄酸类似物,因量少未得到最终的结构确定。结论化合物11为一新化合物,命名为波叶苷(rheoside),化合物13a和13b系首次从该植物中分得。

Chemical constituents of Rheum emodi

Objective To isolate and determine the structures of chemical constituents from the roots and rootstalks of Rheum emodi. Methods The chemical constituents were isolated and purified by silica gel and polyamide column chromatography. Chemical methods and spectroscopic methods, such as ~1H-NMR, ~(13)C-NMR, and MS spectra were used for the structure identification. Results Eighteen com-pounds were obtained. Sixteen of them were identified as: chrysophanol (1), physcion (2), β-sitosterol (3), emodin (4), aloe-emodin (5), rheumin (6), daucosterol (7), d-catechin (8), piceatannol (9), piceatannol-4'-O-β-D-glucopyranoside (10), piceatannol-4'-O-β-D-(6"-O-p-coumaroyl)-glucopyranoside (11), chrysophanol-8-O-β-D-glucopyranoside (12), physcion-1 and 8-O-β-D-glucopyranoside (13a and 13b), emodin-8-O-β-D-glucopyranoside (14), and sucrose (15). Meanwhile, one compound structure simi-lar to rhein was detected. Conclusion Compound 11 is a new compound named rheoside, compounds 13a and 13b are obtained from R. emodi for the first time.