| 丹参酮ⅡA磺酸钠注射液对人肝星状细胞的影响 |
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| 引用本文： | 华海婴,郭旭光,杨波,叶启霞. 丹参酮ⅡA磺酸钠注射液对人肝星状细胞的影响[J]. 中药药理与临床, 2007, 23(6): 12-14 |
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| 作者姓名： | 华海婴 郭旭光 杨波 叶启霞 |
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| 作者单位： | 1. 郑州大学医药科学研究院,郑州,450052
2. 河南省食品药品检验所,郑州,450052 |
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| 基金项目： | 河南省科技攻关项目;河南省科技攻关项目 |
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| 摘 要： | 目的:研究丹参酮ⅡA磺酸钠注射液(Sulfotanshinone Sodium Injection,STSI)抗肝纤维化的作用及其机制。方法:分别以MTT比色法检测STSI对人肝星状细胞(HSC)增殖的影响;观察对细胞乳酸脱氢酶(LDH)活性的影响;以流式细胞仪检测STSI对HSC凋亡率的影响。结果:STSI各剂量在24与48h对HSC的抑制率呈现出明显的量效关系;STSI对HSC的抑制作用不是非特异性细胞毒性所致;HSC的凋亡率随药物浓度增加而增加,并具有显著性差异。结论:STSI能抑制HSC的增值并促进其凋亡。
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| 关 键 词： | 丹参酮ⅡA磺酸钠注射液 肝星状细胞 乳酸脱氢酶 凋亡率 |
Effects of sulfotanshinone sodium injection on human hepatic stellate cells |
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| Hua Haiying,Guo Xuguang,Yang Bo,Ye Qixia. Effects of sulfotanshinone sodium injection on human hepatic stellate cells[J]. Pharmacology and Clinics of Chinese Materia Medica, 2007, 23(6): 12-14 |
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| Authors: | Hua Haiying Guo Xuguang Yang Bo Ye Qixia |
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| Abstract: | Objective:To study the effect and mechanism of sulfotanshinone sodium injection (STSI) on against hepatic fibrosis in vitro. Methods: The influence on cell proliferation of STSI were determined by MTT assay , and detected the activity of lactic acid dehydrogenase (LDH). The apoptosis rate of HSC was performed by the flow cytometry. Results: At 24h and 48h after the treatment With STSI ,the inhibition ratio observation showed a dose-and-time -dependent relationship, the depressant effect on HSC by STSI was not caused by non-specificity cytotoxicity, the apoptosis rate of HSC increased in a drug concentration-dependent manner, as show a significant difference. Conclusion: STSI can obviously inhibit proliferation of HSC in vitro, and can raised apoptosis rate of HSC. |
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| Keywords: | Sulfotanshinone Sodium Injection (STSI) hepatic stellate cell (HSC ) lactic acid dehydrogenase (LDH) apoptosis |
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