凋亡基因bcl-xl在骨肉瘤中的表达

目的探讨肿瘤凋亡基因bcl-xl在骨肉瘤发生中的作用。方法采用链霉菌抗生物素蛋白-过氧化酶连接法(SP法)检测27例传统骨肉瘤和22例良性软骨骨瘤中的bcl-xl的表达,表达程度采用半定量方式判定。结果bcl-xl在骨肉瘤中表达的阳性率为100%,软骨骨瘤中表达的阳性率为4.5%,两者比较差异有统计学意义(P<0.01)。结论骨肉瘤不同分化与阳性半定量间无相关性(P>0.05)。bcl-xl蛋白表达与骨肉瘤的发生有关,但与骨肉瘤的分化程度无关。

Expression of Apoptosis Gene bcl-xl in Osteosarcoma

Objective The aim was to study the role of apoptosis gene bcl-xl in the tumorigenesis of osteosarcoma.Methods The expression of bcl-xl was observed by immunohistochemical assay in twenty-seven classical osteosarcoma and twenty-two benign bone tumors.The extent of expression was evaluated by using half-quantitative method.Results The positive rate of bcl-xl expression was 100% in osteosarcoma, 4.5% in benign osteochondroma.There was a difference with statistical significance(P<0.01) between two positive rates.Conclusion No correlation exists between different differentiation and positive grade(P>0.05).The expression of bcl-xl is involved in the tumorigenesis of osteosarcoma, but not associated with the differentiation of osteosarcoma.