9trans,11trans Conjugated Linoleic Acid Inhibits the Development of Azoxymethane-Induced Colonic Aberrant Crypt Foci in Rats |
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| Authors: | Yumiko Yasui Rikako Suzuki Hiroyuki Kohno Shingo Miyamoto Fumiaki Beppu Masashi Hosokawa |
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| Affiliation: | 1. Department of Oncologic Pathology , Kanazawa Medical University , 1-1 Daigaku, Uchinada, Ishikawa, 920–0293, Japan;2. Department of Oncologic Pathology , Kanazawa Medical University , 1-1 Daigaku, Uchinada, Ishikawa, 920–0293, Japan;3. Division of Food Science and Biotechnology, Graduate School of Agriculture , Kyoto University , Kyoto, 606–8502, Japan;4. Graduate School of Fisheries Sciences , Hokkaido University , 3-1-1 Minato, Hakodate, Hokkaido, 041-8611, Japan |
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| Abstract: | ![]()
We investigated the effects of 9trans,11trans (9t,11t)-conjugated linoleic acid (CLA) isomer on azoxymethane (AOM)-induced colonic aberrant crypt foci (ACF) in rats. Male F344 rats were given 2 weekly subcutaneous injections of AOM (20 mg/kg bw) to induce colonic ACF. They also were fed a diet containing either 0.01%, 0.1%, or 1% 9t,11t-CLA for 4 wk starting 1 wk before the first dosing of AOM. The group that received a diet supplemented with 9t,11t-CLA had a significantly lower number of ACF/colon in comparison to the AOM alone group in a dose-dependent manner up to 0.1%. Furthermore, treatment with 9t,11t-CLA induced apoptosis and suppressed cell proliferation activity in the non-lesional crypts. The downregulation of cyclooxygenase-2 and cyclin D1 and the activation of peroxisome proliferators activated receptor γ were observed in the colonic mucosa of rats fed a diet supplemented with 9t,11t-CLA. Our findings thus provide some novel insight into the chemopreventive effect of 9t,11t-CLA against preinitiation as well as postinitiation stages of colorectal carcinogenesis. |
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