| RGD蛛丝蛋白基因工程菌高密度发酵条件的研究 |
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| 引用本文： | 李敏,涂桂云,黄智华,黄曦.RGD蛛丝蛋白基因工程菌高密度发酵条件的研究[J].生物医学工程学杂志,2005,22(6):1206-1209. |
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| 作者姓名： | 李敏 涂桂云 黄智华 黄曦 |
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| 作者单位： | 福建师范大学,生物工程学院,福州,350007 |
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| 基金项目： | 国家自然科学基金资助项目(30370414)，福建省重大科技资助项目(2001F006) |
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| 摘 要： | 蜘蛛丝因其独特的机械特性,成为自然界性能优良的天然蛋白质纤维。基因工程技术是获取蛛丝蛋白的有效途径。为规模化制备重组RGD-蛛丝蛋白基因工程菌pN SR-16表达产物,通过摇瓶培养确定pN SR-16生长和表达的最优化条件,并在此基础上进行分批补料高密度培养。控制碳源、氮源的流加,发酵液溶解氧浓度和菌体比生长速率,使pN SR-16最终发酵菌体OD600达到57.15,重组丝蛋白表达产物占总蛋白量的20.8%。
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| 关 键 词： | 重组蛛丝蛋白 工程菌 高密度发酵 |
| 收稿时间： | 2004-01-08 |
| 修稿时间： | 2004-01-082004-05-31 |
Study on the Conditions of High Density Fermentation for the Engineering Bacterial Strain with RGD Spider Silk Protein Gene |
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| Li Min,Tu Guiyun,Huang Zhihua,Huang Xi.Study on the Conditions of High Density Fermentation for the Engineering Bacterial Strain with RGD Spider Silk Protein Gene[J].Journal of Biomedical Engineering,2005,22(6):1206-1209. |
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| Authors: | Li Min Tu Guiyun Huang Zhihua Huang Xi |
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| Institution: | College of Bioengineering.Fujian Normal University, Fuzhou 350007,China |
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| Abstract: | Spider silk becomes the protein fibroin with excellent character in the natural world because of its unique mechanical properties.The genetic engineering technique is an effective method to procure the spider silk protein.In order to obtain RGD-spider silk protein gene recombinant engineering strain pNSR-16 on a regular scale,we determine the optimal conditions of the growth and expression of pNSR-16 through culture in laboratory,and based on these,study the fed-batch high density culture.We control the flow of carbon-nitrogen,the dissolved oxygen concentration and the cell specific growth rate,which make the final cell density and concentration of recombinant protein of total protein arrive at OD_(600) 57.15 and 20.8%,respectively. |
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| Keywords: | Recombinant dragline protein Engineering strain High density fermentation |
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