血红素氧合酶-1减轻老龄大鼠供肝缺血再灌注损伤

目的诱导血红素氧合酶-1,提高老龄大鼠供肝的抗氧化能力,观察其能否减轻老龄大鼠供肝缺血再灌注损伤。方法　老龄SD大鼠为供者,成年SD大鼠为受者,随机分为实验组和对照组。实验组:供者供肝切取前24 h腹腔注射血晶素;对照组:供者供肝切取前24 h腹腔注射生理盐水。受者肝移植后3、6、12、24、48 h分别取移植肝组织,检测抗氧化酶、抗氧化剂、凋亡细胞和凋亡因子的表达情况。结果　实验组移植肝组织中超氧化物歧化酶(SOD)和维生素E、维生素C含量较对照组明显增加,丙氨酸转氨酶(ALT)则较对照组明显降低;另外,实验组较对照组凋亡细胞数量减少,BCL-2表达增加,半胱氨酸蛋白酶-3(Caspase 3)表达降低。结论诱导血红素氧合酶-1可以减轻老龄大鼠供肝缺血再灌注损伤,其机制与抑制细胞凋亡有关。

Alleviating the ischemia-reperfusion injury in aged liver by induction of heme oxygenase-1

Objective Enhancing the ability of anti-oxidation in aged rat liver by induction of heme oxygenase-1 . observing whether it can alleviate the ischemia-reperfusion injury and discussing the mechanism. Methods Aged rats served as recipients and adult SD rats as donors, and then were randomly divided into experimental group and control group. In the experimental group, Hemin was injected intraperitoneally 24 h before resection of donor liver, while in the control group, normal saline was given. The liver samples in the recipients were collected 3, 6, 12, 24 and 48 h after liver transplantation. Superoxide dismutase (SOD), catalase (CAT), the content of tocopherol (VE), ascorbic acid (VC), ALT, the apoptosis of the cells, and the apoptotic factor were measured after reperfusion. Results In the experimental group, the contents of SOD, VE, VC were higher than in the control group. The levels of ALT, the apoptotic cells and the expression of Caspase 3 were decreased, while the expression of BCL 2 increased in the experimental group as compared with those in the control group. Conclusions Induction of hem oxygenase-1 can alleviate the ischemia-reperfusion injury in aged liver, which might be contributed to the inhibition of the apoptosis.