新型重组人肿瘤坏死因子对大鼠肝细胞及小鼠肝组织中钙稳度的影响

目的;研究新型重组人肿瘤坏死因子（ｒｈＴＮＦ－ＮＣ）对培养大鼠肝细胞内游离Ｃａ＾２＋浓度（「Ｃａ＾２＋」ｉ）及小鼠肝组织中Ｃａ６２＋含量的影响。方法：以Ｆｕｒａ－２／ＡＭ为Ｃａ＾２＋荧光检测,荧光分光光度法测定培养大鼠肝细胞内「Ｃａ＾２＋」ｉ;原子吸收分光光度法测定小鼠肝组织中Ｃａ＾２＋含量。结果：在加入ｒｈＴＮＦ－ＮＣ ３ｈ后,可剂量依赖性地引起培养大鼠肝细胞内「Ｃａ＾２＋」ｉ显著升高。

Effect of rhTNF-NC on calcium homeostasis in cultured rat hepatocytes and mouse livers

Objective: To investigate the effect of rhTNF NC on free cytosolic Ca 2+ concentration (Ca 2+ ] i) in cultured rat hepatocytes and Ca 2+ contents in mouse livers. Methods: Ca 2+ ] i was measured by fluorescence spectrophotometer with Ca 2+ detector Fura 2/AM and Ca 2+ contents in mouse livers were examined by atomic absorption spectrophotometer. Results: rhTNF NC dose dependently and significantly increased Ca 2+ ] i 3 h after it was added into culture medium. rhTNF NC also dramatically increased Ca 2+ contents in mouse livers and induced serious liver injury in mice previously sensitized with D GalN. Calcium antagonists verapamil and nifedipine obviously decreased Ca 2+ contents and attenuated liver injury. Conclusion: Ca 2+ homeostasis in cultured rat hepatocytes and mouse livers is disordered by rhTNF NC. Verapamil and nifedipine can attenuate liver injury induced by rhTNF NC by decreasing Ca 2+ accumulation.