| SET8-shRNA抑制血管平滑肌细胞增殖和迁移 |
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| 引用本文： | 张东雪,张胜雷,张璐,李同妙.SET8-shRNA抑制血管平滑肌细胞增殖和迁移[J].中国动脉硬化杂志,2018,26(2):152-156. |
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| 作者姓名： | 张东雪 张胜雷 张璐 李同妙 |
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| 作者单位： | 河北医科大学第四医院肾内科; |
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| 摘 要： | 目的探讨赖氨酸甲基转移酶SET8对大鼠血管平滑肌细胞(VSMC)增殖和迁移的影响。方法体外分离培养大鼠VSMC,将VSMC随机分为正常对照组、空质粒组、SET8-shRNA组。应用RT-PCR、Western blot检测SET8和钙黏附蛋白E-cadherin mRNA和蛋白的表达,应用MTT法检测细胞增殖能力,应用Transwell实验检测细胞迁移能力。结果 SET8-shRNA组与正常对照组和空质粒组比较SET8表达水平降低(P0.05)。MTT结果显示,第12 h、24 h、36 h、48 h,SET8-shRNA组细胞增殖活性较正常对照组和空质粒组明显减弱(P0.05)。Transwell实验显示,SET8-shRNA组细胞迁移能力减弱(P0.05)。SET8-shRNA组E-cadherin mRNA和蛋白的表达较正常对照组和空质粒组明显增强(P0.05)。结论干扰SET8基因表达能够促进E-cadherin的表达,抑制大鼠VSMC增殖、迁移能力。
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| 关 键 词： | 赖氨酸甲基转移酶 血管平滑肌细胞 细胞增殖 细胞迁移 |
| 收稿时间： | 2017/7/17 0:00:00 |
| 修稿时间： | 2017/9/21 0:00:00 |
SET8-shRNA inhibited proliferation and migration of vascular smooth muscle cells |
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| ZHANG Dong-Xue,ZHANG Sheng-Lei,ZHANG Lu and LI Tong-Miao.SET8-shRNA inhibited proliferation and migration of vascular smooth muscle cells[J].Chinese Journal of Arteriosclerosis,2018,26(2):152-156. |
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| Authors: | ZHANG Dong-Xue ZHANG Sheng-Lei ZHANG Lu and LI Tong-Miao |
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| Institution: | Department of Nephrology, the Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, Hebei, China,Department of Nephrology, the Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, Hebei, China,Department of Nephrology, the Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, Hebei, China and Department of Nephrology, the Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, Hebei, China |
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| Abstract: | |
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| Keywords: | Lysine methyhransferase Vascular smooth muscle cell Cell proliferation Cell migration |
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