卡那霉素致豚鼠耳蜗细胞凋亡及 caspase- 3表达的研究

目的:探讨卡那霉素耳慢性中毒与豚鼠耳蜗细胞凋亡的关系,及耳蜗细胞凋亡是否与凋亡蛋白酶caspase-3信号转导有关。方法:取50只豚鼠随机分为5组,实验组连续14d肌肉注射硫酸卡那霉素,200mg/(kg·d),分别对健康对照组及停药1、3、7、14d组处死豚鼠,处死前检测其ABR的变化,并利用TUNEL法检测耳蜗细胞凋亡情况及免疫组化法测定caspase-3的表达。结果:豚鼠卡那霉素耳慢性中毒后,随停药时间延长,ABR阈值较对照组明显上升,同时耳蜗细胞的凋亡数增多,且愈近底回愈多,愈近顶回则愈少。caspase-3阳性表达增高,与对照组比较差异均有显著性(P<0.01)。结论:卡那霉素耳慢性损害与耳蜗细胞的凋亡有关;耳蜗细胞的凋亡过程中有Caspase-3的激活。

Apoptosis and caspase-3 expression of guinea pigs cochlear cells with kanamycin ototoxicity

Objective To investigate the effect of kanamycin ototoxicity on guinea pigs cochlear cells apoptosis,and relation between signal transportation of caspase 3 and apoptosis of cochlear cellsMethods Fifty guinea pigs were randomly divided into 5 groupsExperiment groups were intramuscularly injected with kanamycin 200 mg/(kg·d) for 14 days consecutivelyGuinea pigs of control group and experiment group 1 d,3 d,7 d,14 d were sacrificed respectivelyAuditory brainstem response (ABR) and cochlea cells apoptosis ...