ATP-MgCl_2对缺血后肝组织保护作用的研究

研究ATP-MgCl_2对缺血后肝组织的作用。方法:家兔全肝缺血30分钟后,分别给予生理盐水、ATP和ATP-MgCl_2处理,术后第1天,第3天和第5天取血测定谷丙转氨酶(ALT)、乳酸脱氢酶(LDH)和碱性磷酸酶(ALP),并分别检测各组肝组织学的改变。结果:术后第1天ATP-MgCl_2组ALT明显低于对照组(P<0.05),而ATP组与对照组相比无显著差别:ATP-MgCl_2及ATP组的LDH和ALP与对照组相比无显著差别。术后24小时超微结构示对照组肝细胞明显水肿、细胞器严重损伤和血窦内微小血栓;而ATP-MgCl_2组细胞轻度水肿,细胞器基本正常,血窦内无微小血栓。结论:ATP-MgCl_2对缺血肝组织确有保护作用,其机制可能是通过改善微循环和减轻细胞损伤而起作用。

The Effect of ATP-MgCl_2 on Hepatic Function Following Experimental Hepatic Ischemia

Aims: To evaluate the effect of ATP-MgCl2 on hepatic function following experimental hepatic ischemia. Methods: Global hepatic ischemia in rabbits was produced for 30 min in 30 rabbits which were divided into 3 groups and then received 15 ml of saline, ATP or ATP-MgC2 respectively. Day 1,3,5 following operation, blood samples were obtained and alanine transaminase(ALT), lactate dehydrogenase(LDH) and alkaline phosphatase(ALP) were examinated. Results: ALT in ATP-MgCl2 group at the first day was significantly lower than that in saline. There is no significant difference in ALT between ATP and saline groups. No significant difference was found among ATP, ATP-MgCl2 and saline groups in LDH and ALP. Twenty-four hours later, hepatic ultrastructure showed substantial swollen hepatic cells, severely damaged organellae and micro-thrombi in the sinusoid in saline group, where as there are slightly swollen cells, near normal organellae and no thrombi in the sinusoid in ATP-MgCl2 group. Conclusions: It is suggested that infused ATP-MgCl2 would produce the beneficial effect on the ischemia liver and the microcirculation and the cell swelling could be improved.