CD基因修饰的神经干细胞对C6胶质瘤细胞的作用

目的研究CD基因修饰神经干细胞(NSCs)对脑胶质瘤细胞的抑制作用、效果及机制,为NSCs在脑胶质瘤治疗中的应用提供理论依据。方法以胎脑作为供体,采用神经球形成法培养NSCs,脂质体介导CD基因转染NSCs,G418筛选。NSCs/CD与C6胶质瘤细胞分组共培养,MTT检测细胞存活情况。结果成功构建CD基因转染NSCs,NSCs/CD对C6细胞生长有明显抑制作用。结论 NSCs是良好的载体,NSCs/CD-5-FC自杀基因治疗系统具有很强的抗肿瘤效果。

Effect of Neural Stem Cells Modified by Cytosine Deaminase Gene on C6 Glioma Cells

Objective To investigate the inhibition effect of cytosine deaminase(CD) gene-modified neural stem cells(NSCs) and the action mechanism,and to provide a theoretical basis for the treatment of glioma.Methods Neurosphere-forming NSCs were isolated from fetal brain.CD gene was introduced into NSCs by liposome-mediated transfection and placed under G418 selection.NSCs/CD and C6 glioma cells were co-cultured and cell survival was assessed with MTT assay.Results CD gene-modified NSCs were successfully constructed.NSCs/CD significantly inhibited the growth of C6 cells.Conclusion NSCs are good carrier cells and NSCs/CD-5-FC suicide gene therapy system has a strong anti-tumor effect.