脐血血管内皮祖细胞的分离和诱导分化 |
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引用本文: | 张志华,杨晨,李宗金,钱冠清,杨仁池,丁顺利,韩忠朝. 脐血血管内皮祖细胞的分离和诱导分化[J]. 中国微循环, 2003, 7(2): 78-81 |
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作者姓名: | 张志华 杨晨 李宗金 钱冠清 杨仁池 丁顺利 韩忠朝 |
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作者单位: | 300020,天津,中国医学科学院,中国协和医科大学血液学研究所实验血液学国家重点实验室. |
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基金项目: | 973项目(001CB5101),攀登计划(95 -专 -10),天津市自然基金重点项目(013803211),医科院长江学者项目资助 |
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摘 要: | 目的 从脐血中分离内皮祖细胞,诱导其向内皮细胞分化,研究内皮祖细胞的生物学特性和诱导分化条件。方法 从新鲜脐血中纯化的CD133^ 细胞接种于添加了VEGF、bFGF、IGF—1的M199培养液中,观察梭形贴壁细胞的出现时间和特异性细胞标志。结果 培养3—4d可观察到梭形贴壁细胞,14d左右可形成索条状结构,贴壁细胞表达血管内皮细胞特异性标志VE-cadherin,vWF,UEA-1和VEGFR-2。结论 脐血中含有内皮祖细胞,在一定的条件下,可分化为内皮样细胞。
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关 键 词: | 脐血 血管内皮祖细胞 分离 诱导分化 生物学特性 血管新生 胚胎发育 小鼠 造血干细胞 |
文章编号: | 1007-8568(2003)02-0078-04 |
修稿时间: | 2002-12-07 |
Isolation and Differentiation of Endothelial Progenitor Cells From Cord Blood |
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Abstract: | Objective To study biological traits of endothelial progenitor cells,endothelial pogenitor cells from cord blood were isolated and induced to differentiate in optimal medium.Methods CD133 + cells selected from fresh cord blood were cultured in M199medium supplemented with VEGF,bFGF,IGF_1.Attached spindle_like cells were detached and labeled with a series of antibodies against endothelial_specific markers.Results Attached spindle_like cells appeared after4days culture and formed cord_like structure by14days.These cells can bind UEA_1and express endothelial_specific markers,including VE_cadherin,vWF and VEGFR_2.Conclusions There are endothelial progenitor cells in cord blood,which can differentiate into endothelial cells. |
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Keywords: | Cord blood CD133 + cells Endothelial progenitor cells Differentiation |
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