Bcl-2和survivin蛋白在胃肠道间质瘤的表达与意义

目的探讨Bcl-2和生存素(survivin)蛋白在胃肠道间质瘤(GIST)的表达及临床意义。方法应用免疫组化SP法和病理图像分析系统检测85例胃肠道间质瘤(良性28例,潜在恶性16例,恶性41例)中Bcl-2和survivin的表达情况,结合临床病理学资料分组比较并进行统计学分析。结果 85例GIST中Bcl-2和survivin表达的平均灰度值在良性组、潜在恶性组、恶性组中依次递减,阳性表达水平逐渐增高。3组两两比较,各组的平均表达水平差异存在统计学意义(P0.05)。在41个恶性病例中,有浸润或转移的亚组的Bcl-2和survivin表达水平显著高于无浸润转移亚组的表达水平(P0.05)。GIST中Bcl-2和survivin表达有显著的正相关性(rs=0.43)。结论 Bcl-2和survivin高表达能够协同促进GIST的恶变和向外浸润转移,检测这两个指标也许有助于判断GIST的良恶性以及预测肿瘤的预后。

Expression and significance of Bcl-2 and survivin in gastrointestinal stromal tumors

Objective Toinvestigate the expression ofBcl-2 and survivin in gastrointestinal stomal tumors（GIST）and their clinicalpathologic significance.Method The expression of Bcl-2 and survivin were detected in 85 cases of GIST by immunohistochemical technique.Futhermore,multi-function precise color-image measure system was used for quantitative analysis.The relationship between expression and clinicalpathologic data was analyzed.Results Among 85 GISTs,the expression of Bcl-2 and survivin in benign,potentially malignance and malignance group were increased gradually.There was a significant difference among the groups of each gene＇s expression,P〈0.05.There was also significant different in the expression of Bcl-2 and survivin between the metastasis subgroup and the non-metastasis subgroup.There was significant positive correlation between Bcl-2 and survivin expression（rs=0.43）.Conclusions The high expression of Bcl-2 and survivin maybe together contribute to the maglinancy,invasion and metastasis of GIST.Detection of these two markers may be useful in judging the malignancy degree and predicting the prognosis of GIST.