Cyclophosphamide affects the dynamics of granuloma formation in experimental visceral leishmaniasis |
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| Authors: | E. Baldo Corrêa J. M. T. Cunha M. M. Bunn-Moreno E. D. Madeira |
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| Affiliation: | (1) Setor de Microscopia Electrônica, Instituto de Microbiologia, Universidade Federal de Rio de Janeiro, Centro de Ciências de Saúde, Bloco I, Cidade Universitária, Ilha do Fundão, 21941 Rio de Janeiro, Brazil;(2) Departamento de Histologia, Instituto de Ciências Biomédicas, Universidade Federal de Rio de Janeiro, Centro de Ciências de Saúde, Bloco I, Cidade Universitária, Ilha do Fundão, 21941 Rio de Janeiro, Brazil;(3) Departamento de Imunologia, Instituto de Microbiologia, Universidade Federal de Rio de Janeiro, Centro de Ciências de Saúde, Bloco I, Cidade Universitária, Ilha do Fundão, 21941 Rio de Janeio, Brazil |
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| Abstract: | ![]()
We observed histopathological and ultrastructural hepatic changes following the intracardiac inoculation ofLeishmania donovani amastigotes into inbred LHC hamsters (group I). Since granuloma formation is known to be T-cell-dependent, we also examined infected hamsters under cyclophosphamide immunosuppressive treatment (group ICy) and evaluated the production of interleukin-2 (IL-2) by their cells. Group I showed more intense hepatocyte and endothelial cell clasmatosis as well as hepatocyte degeneration and necrosis, deposits of connective tissue fibers, granulomas with multinucleated giant cells (MGCs) of foreign-body and Langhans' types and reduced production of IL-2 by spleen cells. In contrast, group ICy hamsters exhibited larger eosinophil and lymphocyte populations within sinusoids and peri-sinusoidal areas but showed no MGCs in granulomas. A striking decline in IL-2 production was noted. These results suggest that cyclophosphamide induces a delay in the natural evolution ofL. donovani-induced granulomatous hepatic inflammation. |
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