四氧嘧啶制作家兔糖尿病模型的观察研究

目的 探讨四氧嘧啶制作家兔糖尿病模型建立的实验条件,评价模型的稳定性.方法 研究禁食时间,四氧嘧啶给药途径和给药剂量,给药后的处理等因素对造模成功率、死亡率及血糖的影响,考察建模后的稳定性.结果 禁食时间、给药途径、给药剂量、给药后的处理对造模影响较大,是影响造模的主要条件.结论 选择禁食6 h的健康家兔,以耳缘静脉注射130 mg/kg的四氧嘧啶,注药后立即给饲料、青菜和水,可以获得成模率高、死亡率小、血糖持续稳定的糖尿病家兔模型.

Observation on rabbit diabetes model produced by alloxan

［Abstract］　Objective　To investigate the experiment condition of rabbit diabetes model produced by alloxan and evaluate stability of the model.Methods　Impacts of fasting time, alloxan route of administration, dose, and different handling ways of after administration on the model success rate, mortality, blood glucose were studyed in 48 rabbits. Results　Fasting time, administration route, dose and the treatment after administration were the main conditions which affect the molde. Conclusion　By selecting healthy rabbit after fasting 6 hours and injecting 130 mg/kg of alloxan from ear vein and immediately feed after injection, the diabetics rabbit model which has hight success rate, low mortality and stable blood sugar can be obtained.